气单胞菌不同菌种胆汁耐受特征分析

  目的  分析气单胞菌不同菌种胆汁的耐受特征，探讨嗜水气单胞菌与其他气单胞菌感染部位不同的原因。  方法  挑取8个种共20株气单胞菌，测定这些菌的胆汁最低抑菌浓度（MIC）和最低杀菌浓度（MBC），并描述常见气单胞菌的在不同胆汁浓度下、厌氧或需氧环境下的生长情况。  结果  气单胞菌不同菌种对胆汁的耐受能力不同，维氏气单胞菌及肠棕气单胞菌对胆汁适应性最好，MIC为9%~11%，MBC为11%~12%；嗜水气单胞菌、豚鼠气单胞菌、中间气单胞菌及双壳类气单胞菌对胆汁适应性较差，MIC为4%，MBC为6%~10%。 达卡气单胞菌不同来源菌株间胆汁耐受有差异。增加气单胞菌的初始菌量，气单胞对胆汁耐受性增加；其中，嗜水气单胞菌对胆汁的耐受性最好。 厌氧环境更利于气单胞菌早期对胆汁的适应。  结论   气单胞菌不同菌种对胆汁的耐受能力不同；初始菌量及氧气影响气单胞对胆汁的耐受。

Bile tolerance characteristics of different Aeromonas species

  Objective  To understand the bile tolerance of different Aeromonas species and explore the reasons for the different infection sites of Aeromonas hydrophila and other Aeromonas.   Methods  A total of 20 Aeromonas strains from 8 species were selected, and the bile minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) to these strains were tested, and the growth curves of common Aeromonas species under different bile concentrations, anaerobic or aerobic environments were described.   Results  Different strains of Aeromonas had different tolerance to bile. A. veronii and A. enteropelogenes were more resistant to bile, with MIC of 9%–11% and MBC of 11%–12%; A. hydrophila, A. caviae, A. media and A. bivalvium were less resistant to bile, with MIC of 4% and MBC of 6%–10%. There were different bile tolerance among different strains of A. dhakensis. Increasing the initial amount of bacteria, the Aeromonas' tolerance to bile would be higher, and A. hydrophila has the best tolerance to bile. The anaerobic environment was helpful for the early adaptation of Aeromonas to bile.  Conclusion  Different strains of Aeromonas had different tolerance to bile; initial amount of bacteria and oxygen affected the tolerance of Aeromonas to bile.