头颈部放疗期间口腔细菌菌群变化及其与放射性口腔黏膜炎相关性研究

目的:探索口腔细菌菌群在头颈部肿瘤放疗期间的变化及其与放射性口腔黏膜炎(ROM)的相关性。方法:获取入组鼻咽癌患者和陪护家属在放疗前和结束时口腔细菌标本，利用高通量测序技术检测。C57BL/6小鼠构建ROM模型，收集照射后第9天和阴性对照组口腔细菌标本；另一批小鼠解剖获取阴性对照组和小鼠照射后第3、5、7、9天舌头组织...

Correlation between changes of oral bacterial flora and radiation-induced oral mucositis during radiotherapy for head and neck

Objective To investigate the correlation between the changes of oral bacterial flora during head and neck radiotherapy and radiation-induced oral mucositis (ROM). Methods The oral bacterial samples of patients with nasopharyngeal carcinoma and accompanying family members were obtained before and at the end of radiotherapy and subjected to high-throughput sequencing. C57BL/6 mice were used to establish the ROM models. On the 9^th day after radiotherapy, oral bacterial samples were collected in the radiotherapy group and the negative control group. On the 3^rd, 5^th, 7^th, and 9^th days post-radiotherapy, the tongue tissues were obtained from another batch of mice in the negative control and radiotherapy groups. Inflammatory factors were detected with PCR and HE staining was performed. Results The oral bacterial diversity of patients after radiotherapy significantly differed from that of patients before radiotherapy and their accompanying family members before and after radiotherapy in Observed species,Chao1, Simpson index (all P<0.05). There was a significant difference in Shannon index between the severe and mild ROM patients (P=0.036). LEfSe analysis showed that patients with severe ROM had higher levels of g_Streptococcus and f_Streptococcus, and lower levels of f_Familyxl, g_Gemini and o_Bacillus. The Simpson index and PCoA results in the oral bacterial samples significantly differed between the negative control and radiotherapy groups (all P<0.05). Conclusions Radiotherapy can disrupt the balance of bacterial flora. The dysregulated oral bacterial flora is closely associated with the aggravation of ROM.