血清外泌体miR-148a联合miR-106a检测用于胃癌筛查的研究探讨

目的:寻找合适的外泌体mi RNA作为肿瘤生物标志物,辅助临床进行胃癌筛查。方法:将mi R-148a及mi R-106a作为潜在标志物,研究两种mi RNA在癌组织及癌旁组织表达的差异性。选取2017年9月至2018年9月在福建省立医院诊治的初诊胃癌术后标本共37对(胃癌组织及癌旁组织)进行组织学mi RNA检测。选取初诊胃癌患者83例为胃癌组,良性病变的患者78例为对照组,全部进行血清外泌体mi RNA检测。结果:与癌旁组织相比,癌组织中mi R-148a表达水平降低,mi R-106a表达水平升高,联合检测2^-△△CP[△CP=CP₍(mi R-148a))-CP₍(mi R-106a))]值降低。与对照组相比,胃癌患者中血清外泌体mi R-106a表达水平降低,联合检测2^-△△CP值升高。血清外泌体联合检测2-△△CP值对胃癌组和对照组鉴别的曲线下面积[AUC为0.844(95%CI:0.782～0.905,P<0.001)],大于mi R-148a及mi R-106a单独检测,其cu...

Detection of serum exosomal miR-148a combined with miR-106a in screening for gastric cancer

  Objective   To search for suitable exosomal microRNAs (miRNAs) as tumor biomarkers to assist in the clinical screening of gastric cancer.   Methods   The differential expression of miR-148a and miR-106a in cancer and para-carcinoma tissues were determined to assess their potential as biomarkers. These two serum exosomal miRNAs were detected to compare their differential expression in gastric cancer patients and a control group. A total of 37 pairs of postoperative specimens (gastric cancer tissue and para-carcinoma tissue) from gastric cancer patients diagnosed and treated in Fujian Provincial Hospital from September 2017 to September 2018 were selected for histological miRNA detection. Eighty-three patients with newly diagnosed gastric cancer were selected as the gastric cancer group and 78 patients with benign lesions were selected as the control group.   Results   Compared with the adjacent tissues, the expression level of miR-148a decreased, the expression level of miR-106a increased, and the value of 2-△△CP (△CP=CP_(miR-148a)-CP_(miR-106a)) decreased in cancer tissues. Compared with the control group, the expression level of serum exosomal miR-106a decreased and the value of 2-△△CP (△CP=CP_(miR-148a)-_CP(miR-106a)) increased in patients with gastric cancer. The area under the curve of 0.844 (95% CI: 0.782-0.905, P < 0.001) of 2-△△CP by the combined detection of serum exosomal miRNAs was greater than that of serum exosomal miR-148a or serum exosomal miR- 106a detected separately in distinguishing the gastric cancer and control groups. The cutoff value of 2-△△CP(△ CP=CP_(miR-148a)-CP_(miR-106a)) was 0.3153, the sensitivity was 91%, and the specificity was 64.1%.   Conclusions   The 2-△△CP value of the detection of serum exosomal miR-148a combined with miR-106a could be useful in screening for gastric cancer.