胆、肠菌群差异性与胆囊胆固醇结石形成关系

目的 基于胆囊胆固醇结石患者胆道菌群、肠道菌群差异性,探讨胆、肠菌群交互与胆囊胆固醇结石形成关系.方法 选择2020年1月至2020年6月由沧州市人民医院诊疗的胆囊胆固醇结石患者42例为研究对象,记入胆固醇结石组;胆囊炎、胆囊息肉诊治等非胆固醇结石患者40例为非胆固醇结石组.采用Illumina测序技术测序并进行细菌O...

The relationship between the difference of biliary and intestinal flora and the formation of gallbladder cholesterol calculus

Objective To discuss the relationship between the interaction of biliary flora, intestinal flora and the formation of gallbladder cholesterol calculus, based on the differences of biliary flora and intestinal flora in patients with gallbladder cholesterol calculus. Methods Forty-two patients with gallbladder cholesterol calculus diagnosed and treated in Cangzhou People' Hospital from Jan. 2020 to Jun. 2020 were selected and included in the cholesterol calculus group. At the same time, forty patients with cholecystitis, gallbladder polyp and other non-cholesterol stones treated in Cangzhou People's Hospital were selected as the non-cholesterol calculus group. Illumina sequencing technology was used for sequencing and OTU identification of bacteria. CHOD-PAP method and NanoDrop 2000 spectrophotometer were employed to quantitatively analyze cholesterol content in stone samples. The positive rate of DNA and the number of bacterial colonies in bile samples were detected by qPCR. The expression levels of IgA, IgG and IgM of bile immunoglobulin were detected by radioimmunoassay kit. Results The results of quantitative analysis of cholesterol gallstones showed that cholesterol content was ≤70% in 3 cases, 70%-89% in 38 cases, and ≥90% in 1 case. The positive rate of bile bacterial DNA in non-cholesterol calculus group was significantly lower than that in cholesterol calculus group (P<0.05). The number of bacterial colonies in non-cholesterol calculus group was much smaller than that in cholesterol calculus group (P<0.001). The results of bile immunoglobulin assay showed that the contents of IgA (P<0.001) and IgM (P<0.05) in the cholesterol calculus group were higher than those in the non-cholesterol calculus group (P<0.05), and the content of IgG in the cholesterol calculus group was slightly higher than that in the non-cholesterol calculus group, without significant difference (P>0.05). The results of bacterial flora analysis showed that Firmicutes and Fusobacteria were the dominant bacterial groups in bile, while Firmicutes, Actinobacteria and Bacteroidetes were the dominant bacterial groups in stool samples. Conclusion The types of bacteria in biliary tract and intestinal tract are similar in patients with cholesterol gallstone and non-cholesterol gallstone, and their relative abundance is different, which is related to the formation factors of cholesterol gallstone.