抗早熟Ⅱ号对青春期大鼠长骨干骺端胰岛素样生长因子1及其受体基因表达的影响

目的观察抗早熟Ⅱ号对青春期大鼠长骨干骺端胰岛素样生长因子1(IGF1)及胰岛素样生长因子1受体(IGF1蛳R)基因表达的影响,探讨抗早熟Ⅱ号对长骨干骺端骨生长的作用机理。方法将青春期SD雌性大白鼠分为2组,对照组喂饲生理盐水1个月,实验组喂饲抗早熟Ⅱ号中药合剂1个月,采用实时荧光RT蛳PCR定量检测技术检测大鼠长骨干骺端IGF1mRNA及IGF1蛳RmRNA表达水平。结果实验组长骨干骺端IGF1基因表达水平较对照组显著下调(t=10.268,P<0.05);两组间IGF1蛳R基因表达水平差异无统计学意义(t=0.110,P=0.914)。结论抗早熟Ⅱ号通过使长骨干骺端软骨细胞IGF1的基因表达水平下调,从而抑制长骨生长,延缓骨骼成熟。

Effect of Chinese herbal medicine on IGF1 and IGF1- R gene expression on metaphysis in pubertal rats

Objectives To observe the effect of Chinese herbal medicine on insulin-like growth factor 1(IGF1)and insulin-like growth factor 1 receptor (IGF1-R) gene expression on metaphysis and explore the mechanism of Chinese herbal medicine on bone growth.Methods Pubertal female Spraue-Dawley rats were randomly divided into two groups.The study group was fed with Chinese herbal medicine for 30 days while control group was fed with normal saline.IGF1 and IGF1-R mRNA expression in metaphysis were measured with real-time RT-PCR.The parameters were analyzed with t test with SPSS 11.0 software.Results The IGF1 mRNA expression was down regulated (t = 10.268,P < 0.05) in the study group.But,the difference of IGF1-R mRNA expression between two groups was not significant(t = 0.110,P = 0.914).Conclusions Chinese herbal medicine can down regulate IGF1 mRNA expression in cartilage cells of metaphysis,thus it might inhibit the bone growth and delay the maturation of the bones.