| 阴道白假丝酵母菌基因型与体外药物敏感性研究 |
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| 引用本文： | 祁文瑾,许妙玲,李白鸾. 阴道白假丝酵母菌基因型与体外药物敏感性研究[J]. 中国全科医学, 2011, 14(14) |
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| 作者姓名： | 祁文瑾 许妙玲 李白鸾 |
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| 作者单位： | 昆明医学院第一附属医院妇产科,云南省昆明市,650032 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的 研究不同基因型阴道假丝酵母菌对克霉唑、咪康唑和制霉菌素的体外药物敏感性.方法 以特异引物PCR法(INT-PCR)对151株致病白假丝酵母菌进行基因分型,参考美国临床和实验室标准协会(CLSI)抗真菌药敏实验标准(M27-A方案)以微量稀释法检测不同基因型菌株的药物敏感性.结果 根据扩增条带中是否存在450 bp和840 bp片段可将白假丝酵母菌分为A、B、C 3种基因型;克霉唑和咪康唑对A基因型菌株的最小抑菌浓度(MIC)值明显高于C基因型菌株,差异有统计学意义(P＜0.05);制霉菌素对不同基因型菌株的MIC值间差异无统计学意义(P＞0.05).结论 不同基因型白假丝酵母菌株对抗真菌药物的敏感性有明显差异,这种差异可能是临床上假丝酵母菌性外阴阴道炎治疗效果存在个体差异的原因之一.
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| 关 键 词： | 假丝酵母菌性外阴阴道炎 白假丝酵母菌 基因 微生物敏感性试验 |
Genotypes and In-Vitro Antifungal Susceptibility of Candida Albicans Isolated from Vagina |
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| QI Wen-jin,XU Miao-ling,LI Bai-luan. Genotypes and In-Vitro Antifungal Susceptibility of Candida Albicans Isolated from Vagina[J]. Chinese General Practice, 2011, 14(14) |
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| Authors: | QI Wen-jin XU Miao-ling LI Bai-luan |
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| Affiliation: | QI Wen-jin,XU Miao-ling,LI Bai-luan.Department of Obstetrics & Gynecology,the Frist Affiliated Hospital of Kunming Medical College,Kunming 650032,China |
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| Abstract: | Objective To explore the in-vitro antifungal susceptibility of Candida albicans(Ca) isolates representing different genotypes.Methods A total of 151 Ca isolates were genotyped by polymerase chain reaction with INT primers(INT-PCR).The antifungal susceptibility was assessed using a broth microdilution method based on the guidelines(document M27-A) issued by the Clinical and Laboratory Standards Institute(CLSI) of the United States.Results All Ca strains were divided into three genotypes(A,B,and C) based on t... |
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| Keywords: | Vulvovaginal candidiasis Candida albicans Genotype Microbial sensitivity tests |
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