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| 双向凝胶电泳分析脾脏蛋白组学的方法研究 | |
| 引用本文： | 邱芳华,饶卫农,陈求刚. 双向凝胶电泳分析脾脏蛋白组学的方法研究[J]. 中国热带医学, 2011, 11(1): 10-12 |
| 作者姓名： | 邱芳华 饶卫农 陈求刚 |
| 作者单位： | 1. 广州医学院研究生课程进修班,广东,广州,510000;中山大学附属第一医院检验医学部,广东,广州,510080 2. 广州市中医院检验科,广东,广州,510000 3. 中山大学附属第一医院检验医学部,广东,广州,510080 |
| 摘 要： | 目的优化双向凝胶电泳的实验步骤，总结一套适用脾脏组织的双向凝胶电泳技术。方法对双向电泳实验中的关键环节：蛋白沉淀方法；胶条PH的选择；聚焦条件等进行研究与优化，考马斯亮蓝染色后进行凝胶图像比较。结果Cleaningup沉淀蛋白，聚焦电压达到11000伏小时（110kvhs），可以得到多达1000个蛋白点的2Dgel（two—dimensionalpolyacrylamidegel）。结论建立了脾脏双向电泳的具体方法，得到分辨率高重复性好的2Dgel，为脾脏的蛋白质组学研究奠定了基础。 |
| 关 键 词： | 脾脏 蛋白质组学 双向电泳 |
Establishment of two-dimensional polyacrylamide gel electrophoresis techniques for analysis of spleen tissues |
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| QIU Fang-hua,RAO Wei-nong,CHEN Qiu-gang. Establishment of two-dimensional polyacrylamide gel electrophoresis techniques for analysis of spleen tissues[J]. China Tropical Medicine, 2011, 11(1): 10-12 | |
| Authors: | QIU Fang-hua RAO Wei-nong CHEN Qiu-gang |
| Affiliation: | QIU Fang-hua,RAO Wei-nong,CHEN Qiu-gang(The First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,Guangdong,P.R.China) |
| Abstract: | Aim To establish a set of two-dimensional polyacrylamide gel electrophoresis(2-DE) techniques for proteomic analysis of spleen tissues.Methods Different methods or condition for key procedures of 2-DE were adjusted and optimized,including sample preparation,choice of IPG PH,condition of iso-electronic focusing(IEF).Comparison of gel electrophoresis maps after coomassie staining was carried out.Results High-resolution results with repetitiveness were obtained at condition below:7mmol /L urea,2 mol /L thisure... |
| Keywords: | Spleen Proteomics Two-dimensional polyacrylamide gel electrophoresis(2-DE) |
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