| 一种用于芯片质控的通用序列标签寡核苷酸探针的设计 |
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| 引用本文： | 杨琼,沈年汉,蔡翠霞,周珏宇,李凌,郑文岭.一种用于芯片质控的通用序列标签寡核苷酸探针的设计[J].中国热带医学,2011,11(1):39-42. |
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| 作者姓名： | 杨琼 沈年汉 蔡翠霞 周珏宇 李凌 郑文岭 |
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| 作者单位： | 南方医科大学基因工程研究所,广东,广州,510515 |
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| 基金项目： | 广东省医学科研基金，广州市科技计划项目 |
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| 摘 要： | 目的设计一种带有通用序列标签的寡核苷酸探针以应用于寡核苷酸芯片点样的质量控制。方法以HIV寡核苷酸探针作为核心序列，在其一端或两端连接上多种通用序列标签（usz）构建新型探针。然后打印芯片，以Cy5标记的通用引物（Cy5-UP，与UST互补）进行杂交，筛选出5条荧光信号强的探针，重新打印芯片。再分别以不同浓度梯度的、Cy5标记的通用引物（Cy5-UP）和随机引物（Cy5-RP）与芯片杂交，比较两者杂交效率。结果Cy5-UP的杂交结果显著优于Cy5-RP的杂交结果，特别是在较低浓度时。结论利用Cy5标记的通用引物检测探针上的通用序列标签，可以为芯片点样的质量控制提供一种更有效的方法。
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| 关 键 词： | 寡核苷酸芯片 质量控制 探针设计 |
Design of universal sequence-tagged oligonucleotide probes for spot quality control of microarrays |
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| YANG Qiong,SHEN Nian-han,CAI Cui-xia,ZHOU Jue-yu,LI Ling,ZHENG Wen-ling.Design of universal sequence-tagged oligonucleotide probes for spot quality control of microarrays[J].China Tropical Medicine,2011,11(1):39-42. |
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| Authors: | YANG Qiong SHEN Nian-han CAI Cui-xia ZHOU Jue-yu LI Ling ZHENG Wen-ling |
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| Institution: | YANG Qiong,SHEN Nian-han,CAI Cui-xia,ZHOU Jue-yu,LI Ling,ZHENG Wen-ling Design of universal sequence-tagged oligonucleotide probes for spot quality control of microarrays.YANG Qiong,et al.(Institute of Genetic Engineering,Southern Medical University,Guangzhou 510515,Guangdong,P.R.China)(Institute of Genetic Engineering,P.R.China) |
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| Abstract: | Aim To design an universal sequence-tagged oligonucleotide probes for spot quality control of oligonucleotide microarrays.Methods HIV oligo probes used as core target sequences were linked with several universal sequence tags(USTs) at their one or both ends,and were then printed onto the slides.Cy5-labeled universal primers(Cy5-UP)which were complementary to the USTs were hybridized to the arrays.Five probes with stable strong signals were selected,printed again and hybridized with Cy5-UP and Cy5-labeled ra... |
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| Keywords: | Oligonucleotide microarrays Quality control Probe design |
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