前列腺癌组织中PCNA、MVD表达与彩超血流检测的研究

目的：探讨前列腺癌组织中PCNA、MVD表达与彩超血流检测及临床病理关系。方法：对27例前列腺癌，29例前列腺增生症进行彩色多普勒超声（CDFI）检测，将前列腺内动脉血流显示情况进行分级，计测阻力指数（RI）；采用免疫组化（SP）法检测相对应标本内增殖细胞核抗原（PCNA）、微血管密度（MVD）的表达。结果：在前列腺癌中，PCNA、MVD表达强度与血流分级和病理分化有良好相关性，随血流分级增大和病理分化程度减低呈上升趋势, 转移癌RI、PCNA、MVD高于无转移者。前列腺增生症中PCNA、MVD表达强度、血流分级、RI均明显低于前列腺癌组织，两组各项指标间有显著性差异（P＜0.05）。结论：前列腺癌组织中PCNA、MVD表达与血流检测，可从不同角度反映前列腺癌血管生成情况，为临床诊治和预后评估提供信息。

PCNA, MVD expression in prostate cancer and CDFI flow detection

Objective: To assess the relationship between the proliferation cell nuclear antigen(PCNA), microvascular density(MVD) expression and CDFI flow detection with clinical pathology in prostate cancer. Method: In 27 cases prostate cancer and 29 cases hyperplasia, the flow signal were detected by Color Dopper flow imaging(CDFI), and the grading of artery flow in the prostate and the resistance index(RI) was recorded, and then the MVD and PCNA expression in the relevant sample was detected by immune histochemistry technique(SP). Results: The PCNA, MVD expression in cancer group was well correlated to flow grading and the pathological differentiation, and in the high grade tumor and poor differentiation ones, the MVD, PCNA expression tend to increase. RI, PCNA, MVD in metastatic cases were stronger than those in non-metastatic cases. In prostate hyperplasia group, PCNA, MVD expression and flow grading and RI were more lower than those in the cancer group, which have significant difference between the two groups(P<0.05). Conclusions: The blood flow detection by CDFI and the MVD, PCNA expression can effectively reflect angiogenesis in prostate cancer, will help to clinical therapy planning and predict prognosis.