| 血红素加氧酶在局灶性脑缺血再灌注大鼠大脑组织表达测定 |
| |
| 引用本文： | 孙红,白永新.血红素加氧酶在局灶性脑缺血再灌注大鼠大脑组织表达测定[J].实用中医内科杂志,2012(2):17-18,46. |
| |
| 作者姓名： | 孙红 白永新 |
| |
| 作者单位： | 辽宁中医药大学附属第二医院康复病房;辽宁中医药大学 |
| |
| 摘 要： | 目的]测定血红素加氧酶(HO)在局灶性脑缺血再灌注大鼠大脑组织表达。方法]使用随机平行对照方法,将SPF级雄性大鼠20只随机分为两组,缺血组15只参照Zea-logue方法造大鼠大脑中动脉缺血2h再灌注24h模型,假手术对照组5只采用同样方法暴露颈动脉后缝合切口。观察脑组织形态结构,测定HO-1表达。结果]假手术组脑组织形态结构正常,模型组大鼠大脑缺血区组织明显水肿。HO-1表达相同部位缺血组明显高于假手术组(P<0.01)。结论]局灶性脑缺血再灌注大鼠大脑血HO升高,HO-1参与了局灶性脑缺血再灌注损伤。
|
| 关 键 词： | 局灶性脑缺血再灌注 大脑中动脉闭塞 血红素加氧酶 神经元氧化损伤 动物模型 血红素代谢 随机平行对照研究 |
A Study on Expression and Significance of HO-1 in Cerebraltissues of Rat after Focal Cerebral Ischemia and Reperfusion |
| |
| Authors: | SUN Hong BAI Yong-xin |
| |
| Institution: | 1.The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032,Liaoning,China; 2.Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China) |
| |
| Abstract: | Objective] To investigate the effects of the expression of HO-1 in cerebral tissues of rat after focal cerebral ischemia and reperfusion.Methods] 2 hours right intraluminal middle cerebral artery occlusion with reperfusion was induced in 20 rats,and the specimen of brain were performed at the point after 24 hours reperfusion;expressions of HO-1were detected by genechip profile and RT-PCR method.Results] Expressions of HO-1 were seen sparsely in the control specimens,and were markly increased afer transient focal cerebal ischemia and reperfusion(P<0.01).Conclusion] Expression of HO-1 is a significant response to transient focal cerebral ischemia/reperfusion and contributes to the early formasion of vasogenic edema that can be observed all parts of brains. |
| |
| Keywords: | Focal cerebral ischemia Middle cerebral artery occlusion Heme oxygenase Neuronal oxidative damage Animal model Heme metabolism Randomized parallel controlled study |
| 本文献已被 CNKI 维普 等数据库收录！ |
