| Sevag法去除黄芪粗多糖中蛋白质成分的研究 |
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| 引用本文： | 夏泉,刘钢,葛朝亮,许杜娟.Sevag法去除黄芪粗多糖中蛋白质成分的研究[J].安徽医药,2007,11(12):1069-1071. |
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| 作者姓名： | 夏泉 刘钢 葛朝亮 许杜娟 |
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| 作者单位： | 安徽医科大学第一附属医院药剂科,安徽,合肥,230022;安徽医科大学药学院,安徽,合肥,230032 |
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| 基金项目： | 安徽省自然科学基金项目(070413067)，安徽省教育厅自然科学研究项目(KJ2007B149) |
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| 摘 要： | 目的研究Sevag法去除黄芪粗多糖中蛋白质成分的效果。方法Sevag法去除蛋白质,以考马斯亮蓝法和苯酚-硫酸法测定黄芪粗多糖应用Sevag法前后蛋白质和多糖含量的变化。结果经过6次Sevag法处理后,黄芪粗多糖冻干粉中70.8%的蛋白质被去除,而多糖组分的含量变化不大,仅下降4.44%。结论Sevag法用于去除黄芪粗多糖中蛋白质成分切实可行,可作为纯化黄芪粗多糖的手段之一。
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| 关 键 词： | 黄芪粗多糖 Sevag法 蛋白质去除 |
| 收稿时间： | 2007-08-07 |
| 修稿时间： | 2007年8月7日 |
Removal of proteins from crude astragalus polysaccharides by sevag method |
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| XIA Quan,LIU Gang,GE Chao-liang,XU Du-juan.Removal of proteins from crude astragalus polysaccharides by sevag method[J].Anhui Medical and Pharmaceutical Journal,2007,11(12):1069-1071. |
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| Authors: | XIA Quan LIU Gang GE Chao-liang XU Du-juan |
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| Abstract: | Aim To study the effect of removing proteins from crude astragalus polysaccharides by sevag method.Methods Sevag method was adopted to remove proteins.Coomassie brilliant blue method and phonel-sulfuric acid method were used to determine the content variation of proteins and polysaccharides before and after Sevag method.Results 70.8% of proteins were removed by sevag method after treatment for 6 times while little change of the content of polysaccharide(only reduced by 4.44%) was found.Conclusion Sevag method can be used effectively to remove proteins from crude astragalus polysaccharide as well as an effective purification method. |
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| Keywords: | crude astragalus polysaccharides sevag method removal of proteins |
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