| 顺铂诱导卵巢癌SKOV3细胞自噬和凋亡的研究 |
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| 引用本文： | 张石云,段振玲,徐琳,韩雪松. 顺铂诱导卵巢癌SKOV3细胞自噬和凋亡的研究[J]. 现代妇产科进展, 2011, 20(1): 45-47 |
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| 作者姓名： | 张石云 段振玲 徐琳 韩雪松 |
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| 作者单位： | 1. 大理学院附属医院妇产科,大理,671000
2. 昆明医学院第一附属医院妇产科 |
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| 基金项目： | 国家自然科学基金资助项目(No:30860304) |
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| 摘 要： | 目的:研究顺铂诱导卵巢癌SKOV3细胞自噬和凋亡的发生情况,并探讨可能的发生机制。方法:顺铂干预卵巢癌SKOV3细胞后,用MDC染色荧光显微镜观察自噬囊泡及流式细胞仪检测自噬率;AnnexinV-FITC/PI双染色流式细胞仪检测凋亡率及TUNEL法检测凋亡指数;Western blot检测自噬蛋白Beclin1,LC3-Ⅱ和凋亡蛋白caspase9,caspase3的表达。结果:顺铂干预SKOV3细胞后,MDC阳性细胞数明显增多;自噬率提高(P<0.05);凋亡率提高(P<0.05);凋亡指数提高(P<0.05);自噬蛋白Beclin1,LC3-Ⅱ和凋亡蛋白caspase9,caspase3表达均增强(P<0.05)。结论:顺铂不仅可诱导卵巢癌SK-OV3细胞凋亡,而且可诱导卵巢癌SKOV3细胞自噬性死亡。
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| 关 键 词： | 卵巢肿瘤 细胞系 顺铂 自噬 凋亡 |
Research on autophagy and apoptosis of ovarian cancer cell line SKOV3 induced by cisplatin |
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| Zhang Shiyun,Duan Zhenling,Xu Lin,et al.. Research on autophagy and apoptosis of ovarian cancer cell line SKOV3 induced by cisplatin[J]. Current Advances In Obstetrics and Gynecology, 2011, 20(1): 45-47 |
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| Authors: | Zhang Shiyun Duan Zhenling Xu Lin et al. |
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| Affiliation: | Zhang Shiyun1,Duan Zhenling2,Xu Lin2,et al.1.Department of Obstetrics and Gynecology,the Affiliated Hospital of Dali College,Dali 671000,2.Department of Obstetrics and Gynecology,the First Affiliated Hospital of Kunming Medical College |
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| Abstract: | Objective：To investigate autophagy and apoptosis and their mechanism in ovarian cancer cell line SKOV3 induced by cisplatin.Methods：Ovarian cancer cell line SKOV3 was intervened with cisplatin,the autophagy was analyzed by fluorescent microscope and flow cytometry（FCM）with monodansylcadaverin（MDC）staining.Apoptosis rate was measured by FCM with AnnexinV-FITC/PI staining,and apoptosis index was tested by TUNEL assays.And expression of pro-Beclin1,pro-LC3-Ⅱ,pro-caspase3 and pro-caspase9 was measured by Western blot.Results：After intervened SKOV3 cells with cisplatin,cells positive stained with MDC increased and autophagic rate was higher than that of control group（P0.05）.Apoptosis rate and apoptosis index in cisplatin group were higher than those in control group（P0.05）.Expression of pro-Beclin1,pro-LC3-Ⅱ,pro-caspase3 and pro-caspase9 was increased in SKOV3 cell lines after intervened by cisplatin（P0.05）.Conclusion：Cisplatin may not only augment autophagy but also induce apoptosis in ovarian cancer cells. |
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| Keywords: | Ovarian neoplasms Cell line Cisplatin Autophagy Apoptosis |
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