| 来曲唑治疗大鼠子宫内膜异位症模型作用及机制的研究 |
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| 引用本文： | 宋淑芳,尹利荣,齐之迎.来曲唑治疗大鼠子宫内膜异位症模型作用及机制的研究[J].现代妇产科进展,2011,20(1):35-38. |
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| 作者姓名： | 宋淑芳 尹利荣 齐之迎 |
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| 作者单位： | 天津医科大学第二医院妇科,天津,300211 |
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| 摘 要： | 目的:探讨第3代芳香化酶抑制剂(aromatase inhibitors,AIs)来曲唑治疗大鼠子宫内膜异位症模型的效果及作用机制。方法:用自体子宫内膜移植方法建立大鼠内异症模型,随机将20只建模成功大鼠分为来曲唑组和盐水对照组,比较治疗前后EM大鼠异位病灶体积的变化;免疫组化法检测异位内膜病灶中细胞色素P450芳香化酶(P450arom)、环氧合酶-2(COX-2)、血管内皮生长因子(VEGF)、增殖细胞核抗原(PCNA)蛋白的表达;末端转移酶介导的缺口末端标记法(TUNEL)检测细胞凋亡情况;放射免疫分析法测定大鼠血清FSH、LH、E2水平。结果:来曲唑组异位病灶体积缩小(P<0.01);异位内膜中P450arom、COX-2、VEGF、PCNA蛋白表达与对照组相比均明显降低(P<0.05);来曲唑组异位内膜细胞凋亡率较对照组明显增加(P<0.01);两组大鼠血清FSH、LH、E2水平无明显差异(P>0.05)。结论:来曲唑治疗大鼠子宫内膜异位症模型有效。来曲唑通过降低异位内膜局部P450arom、COX-2、VEGF蛋白表达及抑制异位内膜增殖并促进凋亡发挥治疗子宫内膜异位症的作用。来曲唑对大鼠血清FSH、LH、E2水平无明显影响。
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| 关 键 词： | 来曲唑 动物疾病模型 大鼠 Sprague-Dawley 子宫内膜异位症 芳香化酶 |
Effect and mechanism of letrozole treating rat endometriosis model |
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| Song Shufang,Yin Lirong,Qi Zhiying.Effect and mechanism of letrozole treating rat endometriosis model[J].Current Advances In Obstetrics and Gynecology,2011,20(1):35-38. |
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| Authors: | Song Shufang Yin Lirong Qi Zhiying |
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| Institution: | Song Shufang,Yin Lirong,Qi Zhiying.Department of Gynecology,the No.2 Hospital of Tianjin Medical University,Tianjin 300211 |
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| Abstract: | ObjectiveTo explore the effect and mechanism of letrozole treating endometriosis(EM)rat models.Methods:Surgically transplanted autologous uterine tissues in ectopic site beside the uterines in rats were used as animal models.Twenty EM rat models were randomly divided into letrozole group and control group.The change of ectopic lesion volume in each group was compared before and after the treatment.Immunohistochemistry was used to detect the expression of P450arom,COX2,VEGF and PCNA in the endometriotic tiss... |
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| Keywords: | Letrozole Animal disease models Rats Sprague-Dawley Endometriosis Aromatase |
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