葡萄胎组织MMPs与TIMPs表达及意义

目的探讨基质金属蛋白酶（MMPs）及其抑制物（TIMPs）在葡萄胎组织中的表达水平及其临床意义。方法采用逆转录聚合酶链反应方法测定35例葡萄胎（6例发生恶变，列为葡萄胎恶变组，29例为葡萄胎未恶变组）和18例正常绒毛组织（正常绒毛组）中MMP-7、MMP-9、TIMP-1及TIMP-2 mRNA表达水平。结果葡萄胎恶变组MMP-9／TIMP-1 mRNA比值高于正常绒毛组和葡萄胎未恶变组（F=3．62；q=3．82、3．57；P〈0．05）；MMP7、MMP-9、TIMP-1、TIMP-2 mRNA在各组中的表达差异无显著性（P〉0．05）。经Pearson相关性分析显示，在所有组织中MMP-9与MMP-7表达呈正相关（r=0．985，P〈0．01）。结论葡萄胎组织中MMP-9／TIMP-1比值对预测葡萄胎恶变有一定的作用，可为临床预防性化疗提供依据。

EXPRESSION OF MMPs AND TIMPs IN HYDATIDIFORM MOLE AND ITS CLINICAL SIGNIFICANCE

Objective To explore the expressions of MMPs and TIMPs in hydatidiform mole and their clinical significance. Methods The mRNA expressions of MMPs and TIMPs were detected by RT-PCR in tissue of hydatidiform mole(n=35) including six malignant transformed mole and normal villi(n=18). Results The ratios of MMP-9/TIMP-1 mRNA expressions were significantly higher in the malignant transformed mole than those in the nonmalignant one and normal villi(F=3.62,q=3.82,3.57,P<0.05).The differences in expressions of MMPs and TIMPs were not significant in all cases(P>0.05).Positive correlation between MMP-7 and MMP-9 expression was noted(r=0.985,P<0.01). Conclusion It is certain that the ratios of MMP-9/TIMP-1 mRNA expression plays a role in predicting malignancy of hydatidiform mole.