检测颈动脉粥样硬化患者血清基质金属蛋白酶的意义

目的：观察颈动脉粥样硬化（CAS）患者血清中基质金属蛋白酶-1（MMP-1）、基质金属蛋白酶-9（MMP-9）及基质金属蛋白酶抑制剂-1（TIMP-1）含量的变化，说明它们与动脉粥样硬化的关系。方法：通过彩色多普勒超声检查及临床判断为CAS患者77例，用高脂饲料加空气干燥术形成家兔颈动脉粥样硬化模型8只。用酶联免疫吸附试验检测CAS患者和家兔模型血清MMP-1、MMP-9及TIMP-1含量，并计数家兔模型血液循环内皮细胞（CEC）水平。结果：CAS患者和家兔模型血清MMP-1、MMP-9水平明显高于正常对照（P〈0．05），而TIMP-1水平则低于正常对照（P〈0．05）。家兔模型造模4周后开始出现CEC明显增多。血清MMP-1与TIMP-1水平呈负相关。结论：CAS患者血清MMP-1、MMP-9及TIMP-1异常代谢可导致血管内皮破坏，与AS形成密切相关。CAS患者检测这三个指标含量有助于从机理上分析CAS的形成原因，同时可用于观察患者的病变程度和疗效评判。

Meaning of serum matrix metalloproteinase assay in carotid atherosclerosis patients

Objective: To observe the level of matrix metalloproteinase-1(MMP-1),matrix metalloproteinase-9(MMP-9)and matrix metallo-proteinase inhibitor-1(TIMP-1) in carotid atherosclerosis(CAS) patients,to explain the relation between them and carotid atherosclerosis(CAS).Methods: Seventy-seven CAS patients were diagnosed by color doppler ultrasound,and eight experimental carotid atherosclerotic rabbit models were induced by high cholesterol diet plus air drying.The level of serum MMP-1,MMP-9,TIMP-1 in CAS patients and experimental carotid atherosclerotic rabbit models were assayed by enzyme linked immunosorbent assay.And the level of blood circulate endothelial cells in rabbit models was assayed. Results: The level of serum MMP-1,MMP-9 in CAS patients and experimental carotid atherosclerotic rabbit models was significantly higher than in the control group(P<0.05).The level of serum TIMP-1 in CAS patients and experimental crotid atherosclerotic rabbit models was lower than in the control group(P<0.05).The level of blood circulate endothelial cells in rabbit models increased obviously after four weeks.The level of serum MMP-1 was negative correlated with serum TIMP-1. Conclusion: The abnormal metabolism of serum MMP-1,MMP-9 and TIMP-1 could destroy blood vessel endothelium.There was a relation between MMP-1,MMP-9,TIMP-1 and CAS.To assay three important indexes in carotid atherosclerosis cases could help us analyze the reason of CAS,observe the degree of pathological changes and judge curative effect.