Protective effect of erythropoietin pretreatment in testicular ischemia-reperfusion injury in rats |
| |
Authors: | Ergur Bekir Ugur Kiray Muge Pekcetin Cetin Bagriyanik H Alper Erbil Guven |
| |
Institution: | Department of Histology and Embryology, Dokuz Eylul University Medical School, Balcova, Izmir 35340, Turkey |
| |
Abstract: | Background/PurposeThis study was designed to investigate the effects of recombinant erythropoietin (EPO), a hormone widely used for treatment of uremic anemia, in rats subjected to testicular ischemia and reperfusion (I/R).MethodsThirty-five male rats were divided into the following: control, sham operated, ischemia (I), I/R, and I/R + EPO groups. In the I group, 2 hours of left unilateral testicular torsion were performed, and in the I/R and I/R + EPO groups, an additional 2 hours of testicular detorsions were performed. The I/R + EPO group was pretreated intraperitoneally with EPO (500 IU/kg) before reperfusion. Testicular tissue samples were examined for biochemical and histopathologic parameters. Apoptotic cells in all testes were detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling technique and caspase 3 immunohistochemistry.ResultsAt histopathologic examination, ischemic changes in primary spermatocytes were noted in all torted testes. Cellular damage and apoptosis were more severe in ischemic groups than the EPO-pretreated group. There were statistically significant differences in tissue biochemical parameters in the I and I/R groups compared with the I/R + EPO group.ConclusionsThe results of the present study suggest that EPO exerts protective effects against I/R injury via the modulation of free radical scavenger's activities, which decreases lipid peroxidation levels and attenuation of apoptosis. |
| |
Keywords: | Apoptosis Erythropoietin Ischemia-reperfusion Testicular torsion Reactive oxygen species |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|