| 人类端粒酶基因反义核酸对恶性肿瘤细胞端粒酶抑制的实验研究 |
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| 引用本文： | 胡杰英,蒋东霞,何琳. 人类端粒酶基因反义核酸对恶性肿瘤细胞端粒酶抑制的实验研究[J]. 河南医学研究, 2003, 12(1): 14-16 |
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| 作者姓名： | 胡杰英 蒋东霞 何琳 |
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| 作者单位： | 河南省肿瘤研究所医学生物室,河南郑州,450003 |
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| 基金项目： | 河南省科技攻关项目资助课题 (0 0 1170 2 0 4) |
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| 摘 要： | 目的 :研究人类端粒酶 (hTR)基因的反义核酸对K5 62细胞、Hep 2细胞端粒酶活性和K5 62细胞凋亡的影响。方法 :采用TRAP法检测K5 62细胞、Hep 2细胞在反义核酸处理前后端粒酶活性的变化以及采用TUNEL法观察反义核酸处理前后K5 62细胞凋亡的变化。结果 :hTR基因反义核酸能有效抑制K5 62细胞和Hep 2细胞的端粒酶活性 ,并且诱导K5 62细胞发生凋亡。结论 :hTR基因反义核酸能显著抑制肿瘤细胞的端粒酶活性 ,为恶性肿瘤治疗提供了一条新的有效的途径
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| 关 键 词： | 端粒酶 细胞 反义治疗 凋亡 |
| 文章编号： | 1004-437X(2003)01-0014-03 |
| 修稿时间： | 2002-10-10 |
Study of antisense oligonucleotide against hTR RNA on the telomerase activity in K562 and Hep-2 cells and apoptosis in K562 cells |
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| HU Jie ying,JIANG Dong xia,HE Lin. Study of antisense oligonucleotide against hTR RNA on the telomerase activity in K562 and Hep-2 cells and apoptosis in K562 cells[J]. Henan Medical Research, 2003, 12(1): 14-16 |
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| Authors: | HU Jie ying JIANG Dong xia HE Lin |
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| Abstract: | Objective: To study the inhibitory effect of human telomerase activity in K562 and Hep 2 cells and the apoptosis by an antisense oligonucleotide against human telomerase RNA(hTR) in K562 cells.Methods: Telomerase activity of K562 and Hep 2 cells with antisense treatment was measured using the TRAP assay and apoptosis was studied by means of TUNEL method.Results: The telomerase activity was effectively inhibited and the apoptosis was induced by the antisense treatment.Conclusion: Antisense oligonucleotide against hTR can effectively inhibit the telomerase activity of tumor cells,and subject is an effective and novel approach for the treatment of malignant cancers. |
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| Keywords: | telomerase cell antisense treatment apoptosis |
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