miR-126对细胞周期的调控及其与肺癌患者预后的关系

目的研究miR-126在肺癌中的表达变化及其与癌细胞周期调控的关系,并分析miR-126表达对肺癌患者术后生存期的影响。方法对肺癌细胞株A549和49例临床肺癌手术标本进行实时定量RT-PCR检测miR-126的表达;A549细胞转染miR-126表达载体,MTT和流式细胞术检测miR-126表达对癌细胞增殖和细胞周期的影响;Kaplan-Meier曲线和log-rank分析方法比较miR-126高、低表达组之间无瘤生存期和总生存期的差别。结果A549肺癌细胞miR-126低表达（2.10±0.67）,转染miR-126表达载体后,miR-126表达升高（12.33±1.67）;转染miR-126后96小时,A549细胞存活率为（68.33±6.67）%,明显低于亲本A549细胞（105.33±8.58）%,差异有统计学意义（P〈0.001）;与亲本A549细胞比较（G0/G1期：37.48±3.28;S期：62.03±5.23）,转染miR-126的A549细胞G0/G1期细胞比例上升（53.67±6.06）,S期下降（43.21±3.75）,差异有统计学意义（P〈0.001）;肺癌组织miR-126表达水平（12.41±4.34）明显低于癌旁肺组织（23.29±6.28）,差异有统计学意义（P〈0.001）;miR-126高表达组无瘤生存期和总生存期均明显高于低表达组（P〈0.01,P〈0.001）。结论 miR-126在肺癌的发生、发展中有重要作用,可能成为肺癌治疗的可选靶点之一。

Effect of miR-126 on cell cycle regulation and prognosis of lung cancer patients

Objective To investigate the expression of miR-126 in lung cancer and the relationship with cancer cell cycle regulation and to analyze the effect of miR-126 expression on postoperative survival of patients with lung cancer.Methods miR-126 expression was detected in lung carcinoma cell line A549 and lung cancer tissues from 49 cases by real-time quantitative RT-PCR; A549 cells were transfected with miR-126 expression vector and cell viability and cell cycle were determined by MTT and flow cytometry,respectively; Kaplan Meier curves and log-rank analysis were used to compare the differences in disease-free survival（ DFS） and overall survival（ OS） between high and low miR-126 expression groups.Results The expression of miR-126 was lower in A549 cells（ 2. 10 ± 0. 67） and after transfection of miR-126 expression vector,the expression level of miR-126 was increased（ 12. 33 ± 1. 67）; The cell viability of A549 cells 96 h after transfection of miR-126 was（ 68. 33 ± 6. 67） %,significantly lower than that of parental A549 cells（ 105. 33 ± 8. 58） %（ P〈 0. 001）; Compared with parental A549 cells（ G0/G1phase： 37. 48 ± 3. 28; S phase： 62. 03 ± 5. 23）,miR-126-transfected A549 cells showed an increase in G0/G1phase（ 53. 67 ± 6. 06） and a decrease in S phase（ 43. 21 ± 3. 75）,P 〈0. 001; miR-126 expression level was significantly lower in lung cancer tissues（ 12. 41 ± 4. 34） than that in lung tissues adjacent to cancer（ 23. 29 ± 6. 28）,P〈 0. 001; DFS and OS were significantly higher in the group with high miR-126 level than those in the low level group（ P〈 0. 01,P 〈0. 001）. Conclusion miR-126 plays an important role in the oncogenesis and development of lung cancer and may be an optional target for lung cancer therapy.