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| HPLC法测定注射用穿琥宁中的有关物质 | |
| 引用本文： | 王德凤,顾一珠,王晖. HPLC法测定注射用穿琥宁中的有关物质[J]. 齐鲁药事, 2005, 24(9): 539-540 |
| 作者姓名： | 王德凤 顾一珠 王晖 |
| 作者单位： | 1. 山东大学药学院,济南,250012 2. 山东大学齐鲁医院,济南,250012 3. 济南第四人民医院,济南,250012 |
| 摘 要： | 目的建立注射用穿琥宁有关物质的测定方法。方法采用反相高效液相色谱法,色谱柱为Phenomenex Luna C18不锈钢色谱柱,流动相为甲醇-0.05%磷酸二氢钾溶液(60∶40),检测波长为251nm,柱温为30℃,流速为1.0ml.min-1。结果及结论:穿琥宁在5.4~86.4μg.ml-1浓度范围内线性关系良好(r=0.9999)。检测限为1 ng。 |
| 关 键 词： | 穿琥宁 高效液相色谱法 有关物质 |
| 文章编号： | 1672-7738(2005)09-0539-02 |
Determination of Chuanhu Ning Injection related substances by HPLC |
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| Wang De-feng,Gu Yi-zhu,Wang Hui. Determination of Chuanhu Ning Injection related substances by HPLC[J]. qilu pharmaceutical affairs, 2005, 24(9): 539-540 | |
| Authors: | Wang De-feng Gu Yi-zhu Wang Hui |
| Affiliation: | Wang De-feng,Gu Yi-zhu~1,Wang Hui~2 |
| Abstract: | Objective A HPLC method for determination of Chuanhu Ning Injection related substances was established.Methods A C_(18) column was used with the mobile phase of methanol-0.05% KH_2PO_4 solution(60∶40) at the detection wavelength of 251nm.Temperature of column was 30℃,the flow rate was 1.0ml·min~(-1).Results and Conclusion The calibration curve was linear in the concentration range of 5.4～86.4μg·ml~(-1)(r=0.9999).The detection limit was 1ng. |
| Keywords: | Chuanhu Ning HPLC related substances |
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