大鼠同种异体原位肾移植模型的建立及并发症的预防

目的为开展器官移植的移植免疫实验研究，建立大鼠同种异体原位肾移植模型，并探讨防止并发症发生的方法。方法Wistar系大鼠为供体，SD系大鼠为受体。采用左侧原位肾移植法，动脉端-端吻合，静脉袖套吻合，输尿管带膀胱瓣与膀胱吻合。结果共进行大鼠肾移植80次，存活73只，7只大鼠死于并发症，手术成功率为91．3％，建立了稳定、实用的大鼠肾移植动物模型。结论大鼠同种异体原位肾移植是一种成功率较高的动物模型。术前准备、术中操作及术后护理对建立稳定的模型有重要影响。加强并发症的预防可提高成功率。

Technique of Orthotopic Allograft Kidney Transplantation in Rats and Prevention of complications

Objective To expand laboratory investigation of organ transplantation and establish a model of renal orthotopic transplantation in rats and to find the ways of preventing the complications during the operation. Methods Wistar rats were served as donors and SD rats served as recipients. Eighty rat renal transplantations were performed with end-side anastomosis of the left renal artery and ureter, end-end sleevelet anastomosis of the left renal vein. Results Eighty rat renal transplantations were performed, seventy-three rats lived and seven rats died from complications, the success rate of operation reached 91.3%, the model of renal transplantation was successfully prepared. Conclusion Orthotopic kidney transplantation in rats with allografts could be performed animal models with a relatively high rate of success. Preparation of pre-operation, operation and post-operation affect the establishment of rat renal transplantation. The rate of success can be increased by the concrete methods.