严重烫伤大鼠胰岛素受体信号传导缺陷的研究

研究３０％体表面积全层皮肤烫伤大鼠伤后胰岛素受体信号传导改变机理。在不同浓度的胰岛素作用下伤后骨肌膜受最大结合容量降低，胰岛素对腺苷酸环化酶活性调节敏感性降低，但反应性正常，比目鱼肌葡萄糖摄取刺激的敏感性及反应性均明显降低，提示烫伤后骨骼肌胰岛抵抗与胰岛素刺激葡萄糖的跨膜转运的信号传递障碍有关。

Studie on Defects of Insulin Receptor Mediated Signal Transmembrance in Severely Scalded Rats

To clarify the possible impaired pathway of signal transmembrance of insulin resistance in the rats with scalding of 30% TBSA, the binding activity of insulin receptor and effect of insulin stimulated adenylale cyclase activity were evaluated. Glucose transport activity was also determined by measuring the 2 deoxyglucose uptake of soleus muscle at various concentrations of insulin. These results showed that after burn injury: (1) The binding capacity of insulin receptor at various submaximal concentrations of insulin was significantly decreased. (2) The sensitivity of inbibition of insulin to adenylate cyclase was obviously reduced, but there was no apparent differences in the maximum inhibiting ability (responsiveness). (3) Both sensitivity and responsiveness of insulin stimulated 2 deoxyglucose uptake in soleus muscle were apparently impaired. In conclusion, it is suggested that the major postreceptor defect pathway may be located on impaired insulin stimulated glucose transport after burn injury. There may be a normal transmembrance signaling of insulin on regulating adenylate cyclase activity in skeletal muscle.