Immunolocalization of types I, II, and X collagen in the tibial insertion sites of the medial meniscus |
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Authors: | Jizong Gao |
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Institution: | Sports Medicine, Faculty of Health Sciences, Link?ping University, Link?ping, Sweden, SE
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Abstract: | The medial meniscus of the rabbit knee joint attaches to the tibial plateau via anterior and posterior insertions. Intact
meniscal tibial insertions are essential for meniscal function. In the present study the distributions of types I, II, and
X collagen in meniscal tibial insertions were investigated by indirect immunohistochemistry in a rabbit model. Four tissue
zones were histologically identified in the anterior insertion site, including the ligamentous zone, uncalcified and calcified
fibrocartilaginous zones and bone; the ligamentous zone was not observed in the posterior insertion site. Labeling for type
I collagen was found to be strong in the ligament tissue and bone, and weak in the fibrocartilages which were also labeled
for type II collagen. Tissues positive for different types of collagen overlapped and formed an irregular interface with various
angles and depths, especially at the interface between the calcified fibrocartilage and bone. Positive labeling for type X
collagen was identified only in the calcified fibrocartilage zone. The coexistence of types I and II collagen in the meniscal
tibial insertions may indicate that this structural unit is subjected to both compressive and tensile loads. Type X collagen
may play a role in maintaining the calcifying status of this tissue zone, so that its mechanical stiffness is kept between
that of uncalcified fibrocartilage and hard bone. Restoration of the insertional structure including the distinct collagen
distribution should be considered for a functional meniscal substitution.
Received: 22 June 1999/Accepted: 27 September 1999 |
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Keywords: | Collagen Meniscal ?insertions Knee joint Immunohistochemistry |
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