黄连生育期间可溶性糖和氨基酸含量动态的研究

目的:通过研究黄连生育期间可溶性糖和游离氨基酸的变化动态规律,为制定黄连规范化栽培措施提供理论依据。方法:黄连游离氨基酸含量测定用茚三酮比色法,黄连可溶性糖含量测定使用硫酸蒽酮水合热法。结果与结论:黄连生育前期(1~2年)各器官的氨基酸和可溶性糖含量低,黄连生育中期(3~4年)的氨基酸和可溶性糖含量显著上升,生育后期(5年)的根茎和花苔的游离氨基酸含量继续上升,须根和叶游离氨基酸含量下降;除了花苔可溶性糖含量呈现上升趋势外,根茎、叶、须根可溶性糖含量呈现下降趋势。在同年生的黄连中,不同物候期间黄连植株各器官的游离氨基酸和可溶性糖含量随着生长中心的转移而发生规律性变化。

Dynamic change of soluble saccharides and amino acids during the growing period of Coptis chinensis

Objective: To study the changes of soluble saccharides and amino acids during the growing period of Coptis chinensis. Method: Amino acid contents were estaimated by Indene tri-ketone colorimetric method; saccharide contents were determinted by anthracene ketone method. Result and conclusion: The contents of soluble saccharides and amino acids in different organs were low in plants of 1-2 years old. And they were increased in plants of 3-4 years old. At maturation (5 years), the contents of amino acids kept on increasing in rhizome and flower stalk, while decreased in fibre and leaves, and the contents of soluble saccharides showed the downtrend in rhizome, leaves and fibre, except the uptrend in flower stalk.