寻常型银屑病不同证型蛋白组学表达的比较研究

目的 对寻常型银屑病(血热证、血瘀证、血虚证)患者与正常人差异蛋白进行分析,探求三证之间差异性表达蛋白,从蛋白质组学角度揭示三证之间的差异.方法 取寻常型银屑病血瘀证、血虚证、血热证患者的血清,应用蛋白提取、定量、双向电泳技术、质谱鉴定和数据库检索,寻找出三证之间的差异蛋白和共有蛋白,并将这些蛋白进行组间比较.结果 发现了15种显著性差异蛋白(P<0.05),其中补体C3、凝聚素、血清转铁蛋白、间-α-胰蛋白酶抑制剂H4重链(片段)、载脂蛋白E、 补体C4-B、 甘露糖结合蛋白C及富亮氨酸-α-2-糖蛋白在血虚型银屑病患者血清表达水平高于血热型和血瘀型(P<0.05).纤维蛋白原β链、血液接合素、纤维蛋白原γ链、HPX蛋白、α-抗胰蛋白酶、甲状腺素转运蛋白及载脂蛋白Ⅳ在血热型银屑病患者血清中的表达水平高于血瘀型患者(P<0.05).共同蛋白有5种(P<0.05),分别为血液结合素、纤维蛋白原γ链、HPX蛋白、α-抗胰蛋白酶、载脂蛋白A-Ⅳ.结论 寻常型银屑病(血热证、血瘀证、血虚证)血清中存在表达差异性蛋白质,可能与寻常型银屑病的发病机制和银屑病不同证型的物质基础有关.

Comparative Research on the Proteomics in Different Syndrome of Psoriasis Vulgaris

Objective The differential proteins between patients with psoriasis vulgaris (blood heat, blood stasis and blood deficiency syndromes) and healthy people were analyzed and investigated. The three types of syndromes were elaborated from proteomics. Methods The serum of psoriasis vulgaris patients with blood stasis, blood deficiency and blood heat syn-dromes was collected. The differential and same proteins of the three trpes of syndromes were screened by protein extraction, quantitative analyses, two-dimensional electrophoresis, mass spectrometry and database searches, and the differential and same proteins between between groups were compared. Results Fifteen significantly differential expressed proteins were found be-tween groups (P<0.05). The expression of serum alexin C3, condensed pigment, serum turned iron protein, inter-alpha-trypsin inhibitors H4 heavy chain (fragment), lipoproteinb E, serum alexin C4-B, seminose combined protein C and rich leucine-al-pha-2-glycoprotein in psoriasis vulgaris patients with blood deficiency were higher than those in patients with blood heat and blood stasis syndroms (P<0.05). The expression of fibrinogen beta chain, blood connexin, fibrinogen gamma chain, HPX pro-tein, α-antitrypsin, thyroid hormone transporters and apolipoprotein IV in patients with blood heat psoriasis were higher than those in patients with blood stasis syndrome (P<0.05). There are five same proteins, hemopexin, fibrinogen gamma chain, HPX protein, Alpha-antitrypsin, apolipoprotein A-IV. Conclusion There are differential expressed proteins in the serum of patients with psoriasis vulgaris (blood heat, blood stasis and blood deficiency syndromes), which may be related to the different patho-genesis of psoriasis vulgaris and the components of different syndrome types of psoriasis.