电针迎香穴对嗅觉功能障碍大鼠胰岛素样生长因子-1的影响

目的 探讨电针迎香穴对嗅觉功能障碍大鼠胰岛素样生长因子-1(insulin-like growth factor 1,IGF-1)的影响.方法 将50只SD大鼠随机分为5组,分别是正常组、嗅觉功能障碍组、嗅觉功能障碍+眶下神经切断组、嗅觉功能障碍+电针组、嗅觉功能障碍+眶下神经切断+电针组,电针干预迎香穴后,运用嗅觉迷宫实验和对大鼠嗅球组织及血液中IGF-1进行ELISA、免疫组织化学等方法进行分析研究.结果 嗅觉功能障碍+电针组大鼠与正常组相比,其嗅觉功能明显改善(P<0.01),其嗅球组织及血液中IGF-1表达明显升高(P<0.05),而嗅觉功能障碍+眶下神经切断+电针组大鼠与正常组相比,其嗅觉功能和嗅球组织及血液中IGF-1表达无明显改善(P>0.05).结论 电针迎香穴对嗅觉功能障碍模型大鼠具有显著干预效应,其作用机制可能是通过三叉神经通路,促进大鼠体内IGF-1的产生,从而有利于嗅觉系统嗅感神经元(ORN)再生,改善嗅觉功能障碍.

Effect of Yingxiang Acupoint on Insulin-like Growth Factors-1 in Olfactory Dysfunction Rats

Objective To investigate the effect of Yingxiang acupoint on insulin-like growth factors-1 (IGF-1) in olfac-tory dysfunction rats. Methods The 50 SD rats were randomly assigned into five groups, normal control group, olfacto-ry dysfunction group, olfactory dysfunction+infraorbital nerve transection group group, olfactory dysfunction+EA group, olfacto-ry dysfunction+infraorbital nerve transection+EA group. After electro-acupuncture intervention at Yingxiang acupoint, the IGF-1 in olfactory bulb tissue and blood of rats was analyzed by using olfactory maze test, ELISA and immunohistochem-istry methods. Results Compared with the normal group, the olfactory function of the olfactory dysfunction+EA group rats were significantly improved (P<0.01), IGF-1 in the olfactory bulb tissue and blood increased significantly (P<0.05). While the IGF-1 in olfactory dysfunction+infraorbital nerve cut+EA group had no significant improvement (P>0.05). Conclusion Elec-troacupuncture Yingxiang acupoint has a significant intervention effect on olfactory dysfunction model rats and its mechanism may be through the trigeminal nerve pathway, promote the production of IGF-1 in rats. It is conducive to the olfactory mucosa olfactory sense (ORN) of neuronal regeneration and improve olfactory dysfunction.