HPLC法测定不同产地龙胆中龙胆苦苷的含量

目的:建立龙胆苦苷含量测定的方法,测定不同产地龙胆中龙胆苦苷的含量,为控制药材质量提供参考。方法:采用Hypersil ODS2 C18色谱柱;以甲醇-水(25∶75)为流动相;流速为1 m L·min-1;检测波长为270 nm,柱温:30℃,测定不同产地龙胆中龙胆苦苷的含量。结果:龙胆苦苷在0.002 15~0.107 5 g·L~(-1)的浓度范围内呈现良好的线性关系;平均回收率为100.07%,RSD为2.32%。结论:该方法快捷、简便、重复性好,通过测定不同产地龙胆药材中龙胆苦苷的含量,为不同产地龙胆质量的控制提供参考。

Determination of Gentiopicroside in Chinese Gentian Root from Different Habitats by HPLC

Objective:To establish a method for the content determination of gentiopicroside,to determine the content of gentiopicroside in Chinese gentian root from different habitats,and to provide reference for quality control of medicinal materials.Methods:Using Hypersil ODS2 C18 chromatographic column,regarding methanol-water (25 ∶ 75) as mobile phase,the flow rate was 1 ml/min;the detection wavelength 270nm,column temperature:30℃C,the content of gentiopicroside in different habitats was determined.Results:The concentration of gentiopicroside in the range of 0.002 15-0.1075 g · L-1 showed a good linear relationship,the average recovery was 100.07％,and RSD was 2.32％.Conclusion:The method is rapid,simple and reproducible.It can provide reference for the quality control of Chinese gentian root from different habitats by measuring the content of gentiopicroside in Chinese gentian root.