密蒙花颗粒对亚慢性毒性实验中SD大鼠血液生化指标的分析

目的 观察密蒙花颗粒对SD大鼠的亚慢性毒性作用及其血液生化指标变化情况.方法 80只SD大鼠随机分为4组(雌雄各半),即空白对照组及密蒙花颗粒低、中、高剂量组,进行为期3个月的亚慢性毒理实验,其中3个给药组分别将密蒙花颗粒23.75 g、45 g、95 g溶于100 mL生理盐水中,灌胃体积为20 mL/kg,空白对照组予以同等灌胃体积生理盐水灌胃,观察各实验组及对照组血常规、谷丙转氨酶、谷草转氨酶、肌酐、尿素、总胆固醇、甘油三酯、葡萄糖、血钾、血钠、血氯、肌酸激酶等指标变化情况,从而进一步评价密蒙花颗粒对SD大鼠血液系统、肝脏、肾脏、心脏等的毒性作用.结果 密蒙花颗粒对SD大鼠血液生化相关指标无不良影响.结论 密蒙花颗粒对SD大鼠血液生化指标无明显慢性毒性反应,可在临床推广应用.

Sub-chronic Toxicity Study of Buddleja Officinalis Granules on Indexes of Hematology and Serum Biochemistry in SD Rats

Objective To observe the chronic toxicity effects of Buddleja Officinalis granules and the changes of indexes about hematology and serum biochemistry in SD rats. Methods 80 SD rats were randomly divided into 4 groups (male and female are fifty-fifty), including the control group and experiment groups. Experiment groups were divided into low, middle and high dose which were fed with 23.75 g, 45 g and 95 g of Buddleja Officinalis granules mixed with 100 mL physiological saline for the three months' subchronic toxicity test. The gavage administration was 20 mL/kg, and the control group was given equivalent physiological saline. The indexes of white blood cell, red blood cell, hemoglobin, hematokrit, Mch, RDW-CV, blood platelet, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase, creatinine, urea, creatine kinase, kalium, serum sodi-um, blood chloride, cholesterol total, glycerin trimyristate,blood glucose were observed in four groups to evaluate the toxic and side effect of buddleja Officinalis granules on blood system, liver, kidney, heart. Results Buddleja Officinalis granules had no bad influence on the indexes of hematology and serum biochemistry. Conclusion Buddleja Officinalis Granules have no chron-ic toxicity on SD rats and can be extended to application in clinic.