人参皂苷Rg1对H2O2诱导的HT22细胞凋亡及胞内Ca2+变化的影响

目的 研究人参皂苷Rg1对H2O2诱导的HT22细胞凋亡及胞内Ca2+浓度变化的影响.方法 以0、6.25、12.5、25、50、100μg/L人参皂苷Rg1预处理细胞24 h,采用Ca2+荧光染料探针Fluo-3/AM负载细胞1 h后,50 mmol/L H2O2刺激细胞,多功能酶标仪测定荧光强度,激光共聚焦显微镜实时监测[Ca2+]i变化,Hoechst 33258染色检测细胞凋亡.结果 H2O2可诱导细胞内Ca2+浓度增加(P<0.01),并增加细胞凋亡率(P<0.01).不同浓度人参皂苷Rg1可呈剂量依赖性的抑制H2O2诱导的HT22[Ca2+]i增加(P<0.01),抑制细胞凋亡(P<0.01),以50μg/L的作用为最强(P<0.01).结论 人参皂苷Rg1可通过降低H2O2诱导的HT22细胞内Ca2+水平的升高,抑制氧化应激引起的细胞凋亡.

Effect of Ginsenoside Rg1 on H2O2 Induced Apoptosis and Intracellular Ca2+Concentration in HT22 Cells

Objective To investigate the effect of ginsenoside Rg1 on H2O2 induced apoptosis and intracellular Ca2+ con-centration in HT22 cells. Methods Ginsenoside Rg1 with 0, 6.25, 12.5, 25, 50 and 100 μg/L were used to pre-processing HT22 cells for 24 h, separately. Ca2+ fluorescent probe Fluo-3/AM was used to load cells for 1h. Then using 50 mmol/L H2O2 stimulate cells. The fluorescence intensity was measured for microplate reader, [Ca 2+]i changes were monitored by scanning confocal microscopy. Apoptosis were tested by Hoechst 33258 staining. Results The Ca2+ concentration (P<0.01) and apoptosis (P<0.01) induced by H2O2 increased significantly. Different concentrations of ginsenoside Rg1 showed dose dependent inhibition in increasing the HT22 [Ca2+]i (P<0.01) and apoptosis (P<0.01) induced by H2O2, and the concentation of 50 μg / L shows strongest effect (P<0.01). Conclusion The ginsenoside Rg1 could inhibit apoptosis of oxidative apoptosis by reducing the cellu-lar Ca2+ level induced by H2O2.