近红外量子点表皮生长因子单克隆抗体荧光探针对金黄地鼠颊黏膜癌变过程的动态可视化荧光成像研究

目的 利用近红外量子点表皮生长因子单克隆抗体荧光探针对由9,10-二甲基1,2苯并蒽(9,10-dimethylen-1,2-benzanthracene,DMBA)诱导建立的金黄地鼠颊黏膜癌变过程进行动态可视化荧光成像研究.方法 (1)用0.5％DMBA丙酮液诱导建立金黄地鼠颊黏膜癌变各期模型;(2)将水溶性近红外荧光量子点(QD800)表面功能性的修饰藕连表皮生长因子受体单克隆抗体(EGFR mAb)后,形成具有靶向功能性的QD800-EGFR mAb荧光探针;(3)将QD800-EGFR mAb经金黄地鼠各期癌变模型尾静脉注射入血液循环系统以靶向适配原理显示其颊黏膜动态癌变过程荧光图像.结果(1)QD800-EGFR mAb能够通过血液循环系统与金黄地鼠颊黏膜癌变细胞上的EGFR特异性的靶向适配并荧光显影;(2)QD800-EGFR mAb显示的荧光图像随着金黄地鼠颊黏膜癌变程度的加深而增强,且能准确地显示癌灶的形状及浸润深度.结论 QD800-EGFR mAb 通过与口腔癌不同癌变阶段癌细胞表面的 EGFR 靶向性适配结合来显示个体化荧光图像,可能对以后临床研治口腔癌的发生发展及转移规律发挥巨大的影响.

Visual Fluorescence Imaging of Golden Hamster Buccal Mucosa Cancerization Process Dynamic by Near Infrared Quantum Dot Epidermal Growth Factor Monoclonal Antibody Fluorescent Probe

Objective The dynamic visualization of fluorescence imaging of hamster buccal mucosa carcinogenesis pro-cess of induced by 9,10-bis (1,2 benzo anthracene (9,10-dimethylen-1, 2-benzanthracene DMBA) were studied by using near infrared quantum dot epidermal growth factor monoclonal antibody fluorescent probe. Methods (1) The buccal mucosa carcino-genesis hamster models were induced with 0.5％ DMBA acetone solution. (2) After forming the water soluble near-infrared flu-orescent quantum dots (QD800) surface functional modification of the epidermal growth factor receptor monoclonal antibody (EGFR mAb), the targeted functional QD800-EGFR mAb fluorescent probe was formed. (3) The buccal mucosa dynamic car-cinogenesis fluorescence images were formed through injection of QD800-EGFR mAb into the golden hamster carcinogene-sis model tail vein. Results (1) QD800-EGFR mAb in blood circulatory system could combine with the EGFR specific target in golden hamster buccal mucosa cancer cells, and the fluorescence imaging lens was visual. (1) The fluorescence images showed by QD800-EGFR mAb was more clear with the deepening of the golden hamster buccal mucosa cancerization degree, and QD800-EGFR mAb fluorescent probe could accurately display the shape of the tumor and the depth of invasion. Conclusion The individual fluorescence images are displayed by QD800-EGFR mAb combined with EGFR target in cancer cell surface at different stages of canceration, which may play an effect on the clinical further research of oral cancer occur-rence development and transfer rules.