“治癌灵”穴位贴敷联合针刺对H22肝癌小鼠Nm23-H1表达的影响

目的:观察"治癌灵"穴位贴敷联合针刺对H22移植性肝癌小鼠肿瘤转移抑制基因Nm23-H1蛋白水平表达的调节作用,探讨该疗法对肿瘤的抑制作用是否与提高Nm23-H1的表达有关。方法:选取健康雄性昆明种小鼠50只,采取完全随机分组法,依照随机数字表法选取10只小鼠,设为正常组,余者造模,经正常饲养7天后,随机分为模型组、穴位贴敷组、针刺组、综合组,每组10只。于雄性昆明种小鼠前肢右腋皮下接种0.2 m L H22肿瘤细胞悬液进行造模。计算瘤重和小鼠肿瘤生长抑制率,采用免疫组化法检测瘤细胞中Nm23-H1的含量。结果:与模型组比较,各组均能抑制瘤体生长(P0.05),明显改善小鼠的生存质量,且综合组优于穴位贴敷组和针刺组(P0.05);与模型组比较,各组均能明显提高Nm23-H1蛋白表达水平(P0.01),综合组优于穴位贴敷组和针刺组(P0.05)。结论:"治癌灵"穴位贴敷及针刺均可提高H22移植性肝癌小鼠Nm23-H1蛋白表达水平,进而抑制癌细胞的生长及肿瘤侵袭转移,改善生存质量,且穴位贴敷联合针刺疗法疗效更佳。

The Influence of "Zhiailing" for Acupoint Application in Combination with Acupuncture on Nm23-H1 of Mice with Liver Cancer

Objective:To observe the regulating function of "Zhiailing" (ZAL) for acupoint application in combination with acupuncture on tumor metastasis suppressor gene Nm23-H1 of mice with H22 transplanted liver cancer,investigate whether the treatment inhabiting tumor is related to the increase of Nm23-H1 expression.Methods:Fifty healthy male Kunming mice were selected and 10 mice of them were chosen into the normal group according to random number table method.The other mice were modeled and fed for 7 days,then randomly divided into the model group,the acupoint application group,the acupuncture group and the integrated group,with 10 mice in each group.These male mice were inoculated with 0.2ml of H22 tumor cell suspension subcutaneously on the right armpit of the forelimb.Tumor weight and tumor growth inhibition rate were calculated,and the content of Nm23-H1 in tumor cells was detected by immunohistochemistry.Results:Compared with the model group,each group could inhibit tumor growth (P ＜ 0.05),and significantly improved the quality of life in mice,and the integrated group was better than the acupoint application group and the acupuncture group (P ＜ 0.05).Compared with the model group,the expression level of Nm23-H1 protein was significantly improved in each group (P ＜ 0.01),and the integrated group was better than the acupoint application group and the acupuncture group (P ＜ 0.05).Conclusion:Both ZAL for acupoint application and acupuncture can improve the Nm23-H1 protein expression of H22 transplanted liver cancer mice,and then inhibit the growth of tumor invasion and metastasis of cancer cells,improve the quality of life,and acupoint application combined with acupuncture therapy has better curative effect.