点腺过路黄免疫调节成分的提取及对小鼠淋巴细胞增殖的影响

目的：观察点腺过路黄提取成分体外对小鼠淋巴细胞增殖的影响。方法：用极性递增的溶剂顺次萃取出全草的脂溶性及水溶性成分，并分别研究它们对小鼠淋巴细胞增殖的影响。结果：氯仿提取部分（10^-10-10^-6g/ml)可显著增强活化的淋巴细胞的增殖，正丁醇提取部分（10^-10-10^-6g/ml)可显著抑制活化的B淋巴细胞的增殖，在10^-6g/ml时可显著增强活化的T淋巴细胞的增殖，而在10^-8-10^-6g/ml时则显著抑制活化的T淋巴细胞增殖。结论：点腺过路黄全草的氯仿及正丁醇部分提取物可能具有免疫调节功能。

The Extraction and Effects on Mouse Lymphocyte Proliferation of Immunoregulation Components from Lysimachia hemsleyana

Objective: To study the effects of extracted fractions from the whole plants of Lysimachia hemsleyana Maxim on the proliferation ability of lymphocytes in mice in vitro. Methods: We extracted the components by using of ethanol, ether from petroleum, chloroform, ethyl acetate and n-butanol, successively then study them on the proliferation of lymphocytes in mice. Results: The n-butanol fraction obviously inhibited the proliferation of spleen lymphocytes induced by LPS, bilaterally regulated the T lymphocyte proliferation induced by ConA. The chloroform fraction markedly enhanced the lymphocytes proliferation induced by ConA or LPS. Conclusion: The results suggested that n-butanol fraction and cholroform fraction from Lysimachia hemsleyana possibly maybe have immunoregulation effects.