| 恙螨RAPD－PCR初步研究 |
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| 引用本文： | 陈景龙,赵彤言,黄翔,林建银,王敦清.恙螨RAPD－PCR初步研究[J].福建医科大学学报,1995(2). |
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| 作者姓名： | 陈景龙 赵彤言 黄翔 林建银 王敦清 |
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| 作者单位： | 福建医学院寄生虫学教研室 |
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| 摘 要： | 通过制备恙螨的基因组ＤＮＡ，利用一组１０个碱基序列的单链随机引物，建立恙螨的随机引物ＤＮＡ多态性分析技术（ＲＡＰＤ－ＰＣＲ），为恙螨的种间、种内、种下、种群之间的分子分类、遗传特性、亲缘关系等研究打基础。本研究筛选出Ｎ０１，Ｎ０５，Ｎ０８，Ｎ１０４个随机引物，较适的缓冲液ｐＨ值，Ｍｇ￣（２＋）浓度和模板ＤＮＡ浓度，以及ＰＣＲ实验参数。
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| 关 键 词： | 恙螨，基因组DNA，电泳，RAPD－PCR |
A Preliminary RAPD-PCR Study on Chigger Mites |
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| Chen Jinglong,Zhao Tongyan,Huang Xiang, et al.A Preliminary RAPD-PCR Study on Chigger Mites[J].Journal of Fujian Medical University,1995(2). |
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| Authors: | Chen Jinglong Zhao Tongyan Huang Xiang |
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| Abstract: | Larvae of chigger mites are the vectors of scrub typhus.There are still many problems and different opinions on differentiating and identifying the chigger mites. In this study.after extracted the genomic DNA from chigger mites,with 10-mer single-stranded DNA primers,the approaches to RAPD-PCR in chigger mites were established.This technique can be used to differentiate and identify the species groups,cryptic species,sibling species,interspecies,intraspecies,subspecies,population,genetic characteristics of chigger mites and their relationship.The primers of N01,N05,N08,N10 were selected in this experiment, the suitable pH(8.3),Mg2+concentration(about2.0mmol/L),template DNA concentration and the parameters of PCR were settled. |
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| Keywords: | chigger mite genomic DNA electrophoresis RAPD-PCR |
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