乳腺浸润性癌血管生成相关因子的表达及其临床意义

目的探讨乳腺浸润性癌血管生成相关因子(VEGF,TGFβ1)的表达与微血管计数(MVC)之间的关系及其临床意义。方法经手术治疗的乳腺浸润性癌62例及12例正常乳腺组织,分别进行HE染色和CD34、VEGF及TGFβ1的免疫组化染色。根据CD34阳性结果计数测定MVC。记录乳腺浸润性癌患者的临床病理特征如年龄、肿瘤大小、病理分型及有无腋淋巴结转移等资料,并将MVC、VEGF和TGFβ1表达结果分别与上述指标进行统计分析。结果乳腺浸润性癌组织MVC值(55.62±11.07)、VEGF和TGFβ1阳性表达率(分别为51.61%和56.45%)均显著高于正常乳腺组织(分别为12.65±5.73,16.67%,16.67%)(P<0.05);MVC值在有腋淋巴结转组(65.53±20.36)高于无腋淋巴结转移组(46.15±16.52),差异有显著性(P<0.01);VEGF和TGFβ1表达的阳性率在有腋淋巴结转移组(分别为68.75%和78.13%)高于无腋淋巴结转移组(均为33.33%),差异有显著性(P<0.05);当VEGF或TGFβ1表达阳性时,MVC值增高(分别为62.82±16.31和59.35±12.76),与VEGF或TGFβ1表达阴性组(分别为51.16±12.53和50.80±15.62)比较差异有显著性(P<0.05);VEGF和TGFβ1之间也存在较明显的相关性(P<0.05)。结论VEGF和TGFβ1相互作用介导了肿瘤血管生成,MVC、VEGF和TGFβ1与淋巴结转移密切相关,可作为判断乳腺浸润性癌预后的参考指标。

Expression of angiogenesis-related factors in invasive breast cancer and its clinical significance

OBJECTIVE: To investigate the relation between expression of angiogenesis-related factors, namely vascular endothelial growth factor (VEGF) and transforming growth factor-beta1 (TGFbeta(1)), and microvessel count (MVC) in invasive breast cancer and analyze its clinical implications. METHODS: VEGF, TGFbeta (1) and CD34 expressions in 62 surgical specimens of invasive breast cancer and 12 normal breast specimens were examined by immunohistochemistry and HE staining. MVC was calculated according to the quantification of positive CD34 expression. Clinicopathological characteristics of the patients including age, tumor size, histological type and auxiliary lymph node metastasis were recorded and compared with the results of MVC VEGF and TGFbeta1 expression and detection. RESULTS: MVC and of VEGF and expressions TGFbeta (1) in invasive breast cancer group (55.62-/+11.07, 51.61%, 56.45%, respectively) were greater than those in the normal control group (12.65-/+5.73, 16.67%, 16.67%, respectively, P<0.05). MVC and the positivity rates of VEGF and TGFbeta (1) expressions were 65.53-/+20.36, 68.75% and 78.13%, respectively, in invasive breast cancer patients with axillary lymph node metastasis, significantly higher than those without metastasis (P<0.05). MVC was correlated with VEGF and TGFbeta (1) expressions in that MVC was significantly higher in patients positive for VEGF and TGFbeta (1) (62.82-/+16.31 and 59.35-/+12.76) than in those negative for their expressions (51.16-/+12.53 and 50.80-/+15.62, P<0.05). Significant correlation was also found between VEGF and TGFbeta (1) expressions (P<0.05). CONCLUSION: The interaction between VEGF and TGFbeta (1) mediates angiogenesis, and MVC and VEGF and TGFbeta (1) expressions are correlated to lymph node metastasis, which may provide reference for prognostic evaluation of invasive breast cancer.