| 上调核型Clusterin基因对A549细胞生物学行为的影响 |
| |
| 引用本文： | 刘士磊,陈海泉,李宗海,李鹤成.上调核型Clusterin基因对A549细胞生物学行为的影响[J].肿瘤,2010,30(2). |
| |
| 作者姓名： | 刘士磊 陈海泉 李宗海 李鹤成 |
| |
| 作者单位： | 1. 复旦大学附属肿瘤医院胸外科,复旦大学上海医学院肿瘤学系,上海,200032
2. 上海交通大学肿瘤研究所癌基因及相关基因国家重点实验室,上海,200032 |
| |
| 基金项目： | 1.国家自然科学基金资助项目(编号:30600756)2.上海市科委科技启明星资助项目 |
| |
| 摘 要： | 目的:观察上调核型Clusterin(nuclear clusterin,nCLU)的表达对人肺腺癌A549细胞生物学行为的影响.方法:构建插入核型Clusterin基因的真核表达质粒,采用脂质体法转染A549细胞,潮霉素B筛选得到稳定转染的细胞株以及对照细胞株; Western 印迹法检测转染前后A549细胞中Clusterin蛋白的表达;CCK-8法检测被转染细胞体外增殖的改变;FCM法检测细胞周期分布的改变;体外迁移和侵袭实验检测细胞体外运动迁移和侵袭能力的变化.结果:nCLU表达的上调,可使A549细胞体外增殖能力明显减慢;G_0/G_1期细胞比例明显增加,由原来的(33.54±2.10)%上升到(63.31±4.30)%;体外迁移及侵袭能力明显减弱(P<0.05).结论:上调nCLU的表达可明显抑制肿瘤细胞的增殖,降低细胞的运动迁移和侵袭能力.
|
| 关 键 词： | 癌 非小细胞肺 细胞增殖 细胞运动 Clusterin基因 |
The effects of up-regulation of nuclear Clusterin gene on the biological behaviors of A549 cells |
| |
| LIU Shi-lei,CHEN Hai-quan,LI Zhong-hai,LI He-cheng.The effects of up-regulation of nuclear Clusterin gene on the biological behaviors of A549 cells[J].Tumor,2010,30(2). |
| |
| Authors: | LIU Shi-lei CHEN Hai-quan LI Zhong-hai LI He-cheng |
| |
| Institution: | LIU Shi-lei1,CHEN Hai-quan1,LI Zhong-hai2,LI He-cheng1 (1.Department of Thoracic Surgery,Fudan University Cancer Hospital,Department of Oncology,Fudan University Shanghai Medical College,Shanghai 200032,China,2.State Key Laboratory of Oncogenes , Related Genes,Shanghai Cancer Institute,Shanghai Jiaotong University,China) |
| |
| Abstract: | Objective:To observe the up-regulation of nuclear Clusterin (nCLU)gene on the biological behaviors of human non-small cell lung cancer cell line A549.Methods:Sense eukaryotic expression vector of nCLU was constructed by cloning the cDNA of nCLU into pIREShyg3 vector. A549 cells were transfected with pIRES-nCLU and pIREShyg3 vectors by lipofectAMINETM 2000 mediation,respectively. Stable transfected cells were selected by hygromycin B screening. CCK-8 assay was performed to evaluate the effect of nCLU over-ex... |
| |
| Keywords: | Carcinoma non-small cell lung Cell proliferation Cell movement Genes Clusterin |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
