生发中心淋巴细胞凋亡与小结树突细胞关系的研究

研究人淋巴结和扁桃体生发中心内的凋亡细胞与小结树细胞之间的关系。方法用ＴＵＮＥＬ和免疫化学方法分别检测凋亡细胞，ＦＤＣ和Ｂｃｌ－２蛋白的表达。结果在生发中心有散在的凋亡细胞且多被巨噬细胞所吴噬；

APOPTOSIS OF B LYMPHOCYTES IN GERMINAL CENTER CORREL-ATED WITH FOLLICULAR DENDRITIC CELLS(FDCs)

Objective\ To study the relationship between the apoptotic cells and follicular dendritic cells (FDCs) in germinal center of human lymph nodes and tonsils. Methods\ TUNEL and immunocytochemical methods have been used to detect the apoptotic cells, FDCs and Bcl 2 protein. Results\ Apoptotic cells displaying in germinal center were almost phagocytozed by macrophages. The FDCs principally located in the light zone of germinal center. They have rich dendritic processes to form dense networks. Bcl 2 protein mainly distributed in the mantle zone of secondary follicles, while germinal center was negative. Conclusions\ FDCs formed in networks in light zone were facilitated to form the contact between centrocytes and FDC, and led to the selection of high affinity centrocytes by rescusing. Apoptosis might be considered as the result of selection process, and lack of Bcl 2 protein might be contributed to apoptosis. However, the Bcl 2 in the mantle zone was assumed to help B cells continue differentiation and inhibit the induction of apoptosis. ;