雷公藤内酯醇抑制A375细胞增殖和迁移及CCR7表达的体外实验

目的观察雷公藤内酯醇在体外抑制人黑素瘤细胞A375增殖和迁移的能力及对细胞表面趋化因子受体CCR7表达的影响情况,初步分析其作用机制。方法用CCK-8比色试剂盒检测雷公藤内酯醇对A375细胞增殖的作用,Transwell微孔隔离小室迁移实验检测雷公藤内酯醇对A375细胞体外迁移能力的影响,Western blot法检测雷公藤内酯醇对A375细胞CCR7蛋白表达的影响。结果雷公藤内酯醇以剂量依赖方式抑制A375细胞的增殖,24h的IC50值为0.20μg/mL;雷公藤内酯醇能显著地抑制A375细胞的体外迁移能力(P0.01),侵袭抑制率约为82.84%;经不同浓度雷公藤内酯醇(0μg/mL,0.2μg/mL和0.4μg/mL)处理后,A375细胞中CCR7蛋白表达呈明显下降趋势(P0.05)。结论雷公藤内酯醇具有抗A375细胞增殖和体外迁移能力的作用,其机制可能与下调其表面趋化因子受体CCR7的表达相关,并且该作用呈一定的浓度依赖性。

The Inhibitory Effects of Triptolide on Cell Proliferation, Migration and the CCR7 Expression of A375 Cells in Vitro

Objective To investigate the inhibitory effects of triptolide on cell proliferation and metastasis of human melanoma cell line A375 and the effects of triptolide on CC-chemokine receptor 7(CCR7) expression, and to discuss the potential anti-tumour mechanisms of TPL in vitro theoretically. Methods The effects of triptolide on the proliferation and migration of A375 cells were detected by the Cell Counting Kit-8(CCK-8)assay and Transwell chamber respectively. The effect of different concentration of Triptolide on CCR7 protein expression of A375 cells was studied by Western blot. Results Triptolide inhibited the proliferation of A375 cells in a dose-dependent way with a 24h IC50 value of 0.20μg/mL. The migration ability of A375 cells was significantly blocked by triptolide with an invasion-inhibition ratio of 82.85%(P<0.01). Triptolide could significantly downregulate the expression of CCR7 on A375 cells in a dose-dependent manner. Conclusion Triptolide could inhibit the cell proliferation and migration of A375 cells in vitro in a dose-dependent way, and the potential mechanism might be related to the downregulation of CCR7 expression of A375 cells.