细胞间粘附分子-1在着床前小鼠胚胎培养液中的表达

为探讨细胞间粘附分子－１（ＩＣＡＭ－１）在着床中的作用和调节，本研究采用昆明种小鼠，获取孕第一天的单细胞胚胎，分别在Ｍ１６培养液和含０．１ｎｇ／ｍｌ浓度白血病抑制因子（ＬＩＦ）的Ｍ１６培养液中培养１２０小时，每２４小时更换培养液，然后，用免疫印迹法测定胚胎培养液中的ＩＣＡＭ－１在蛋白水平的表达。结果：（１）５天的培养液中均有ＩＣＡＭ－１的表达。第一天培养液中的ＩＣＡＭ－１水平稍高，第二天最低，以后逐渐升高，到第五天达最高峰。（２）ＬＩＦ可显著提高胚胎ＩＣＡＭ－１的表达水平。结果表明：小鼠孕早期胚胎可以表达ＩＣＡＭ－１，其表达水平在胚泡期最高。ＩＣＡＭ－１的表达可能与着床过程胚泡的粘附有关。细胞因子ＬＩＦ可促进ＩＣＡＭ－１的表达。

The expression of intercellular adhesion molecule 1 in the early embryo of mouse cultured in vitro

Objective:To study the expression of intercellullar adhesion molecule 1(ICAM 1) and the effect of leukaemia inhibitory factor (LIF) on the expression of ICAM 1 in the early embryo. Design: An experiment study with mouse embryos in vitro. Setting:Institute of Obstetrics and Gynecology Hospital,Institute of Liver Cancer of Zhongshan Hospital,Shanghai Medical University. Methods: Single cell embryos were incubated in M16 medium or M16 containing,0.1 ng/ml LIF for 120 hours.The culture medium was changed each day and the protein level of ICAM 1 in the medium was measured with immunoblotting method. Results:(1)The level of ICAM 1 was higher on the first day than on the second.The level started to increase from the second day,reaching the highest level at the fifth day.(2)LIF improved the expression of ICAM 1 in the embryos. Conclusion:ICAM 1 was expressed in the early embryos of mouse.The level of ICAM 1 is the highest in the stage of blastocyst than in other stage.The expression of ICAM 1 may be related to the attachment of blastocyst during implantation.The expression of ICAM 1 in the embryos can be improved by LIF.