| HPLC测定地耳草中槲皮苷的含量 |
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| 引用本文： | 俞建平,陈勇,郑建宝.HPLC测定地耳草中槲皮苷的含量[J].中国现代应用药学,2009,26(6):499-500. |
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| 作者姓名： | 俞建平 陈勇 郑建宝 |
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| 作者单位： | 1.浙江省药品检验所，杭州 310004；2.浙江中医药大学中药饮片厂，杭州 310009 |
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| 摘 要： | 目的测定地耳草中槲皮苷的含量。方法采用高效液相色谱法,AgilentC18柱（250mm×4.6mm,5μm）,流动相为乙腈-0.05mol·L^-1磷酸二氢钾溶液（19∶81）,检测波长为256nm,流速为1.0mL·min^-1。结果槲皮苷进样量在0.1204～2.408μg内呈良好的线性关系,平均回收率为96.0%,RSD=1.3%（n=6）。结论该方法快速简便,稳定可靠,专属性强。
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| 关 键 词： | 高效液相色谱法 地耳草 槲皮苷 含量测定 |
| 收稿时间： | 1/1/1900 12:00:00 AM |
Determination of Quercitrin in Hypericum japonicum Thunb. By HPLC |
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| YU Jianping,CHEN Yong,ZHENG Jianbao.Determination of Quercitrin in Hypericum japonicum Thunb. By HPLC[J].The Chinese Journal of Modern Applied Pharmacy,2009,26(6):499-500. |
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| Authors: | YU Jianping CHEN Yong ZHENG Jianbao |
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| Institution: | 1. Zhejiang Institute for Drug Control, Hangzhou 310004, China; 2. TCM for Decoction Factory of Zhejiang Chinese Medical University, Hangzhou 310009, China |
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| Abstract: | OBJECTIVE To determine the content of quercitrin in Hypericum japonicum Thunb. by HPLC. METHODS An Agilent C18 column（250 mm×4.6 mm, 5 μm） was used. The mobile was acetonitrile-0.05 mol·L^-1 potassium dihydrogen phosphate（19 ： 81）, and detected at 256 nm with flow rate of 1.0 mL·min^-1. RESULTS A good linearity was observed for quercitrin in the range of 0.120 4-2.408 μg. The average recovery of quercitrin was 96.0% with RSD of 1.3%（n=6）. CONCLUSION The method is convenient, stable, reliable and special for the dermination of quercitrin in Hypericum japonicum Thunb.. |
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| Keywords: | ambroxol hydrochloride HPLC |
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