牛磺酸预处理对大鼠心肌梗死的保护作用

目的观察牛磺酸(taurine,Tau)预处理在大鼠心肌梗死模型中的心脏保护作用。方法 40只健康♂Wistar大鼠随机分为5组:心肌梗死模型组,结扎冠状动脉左前降支造模;对照组,不结扎冠状动脉,其他与模型组同;Tau低剂量组(100 mg·kg-1),Tau中剂量组(200 mg·kg-1),Tau高剂量组(400 mg·kg-1),各腹腔注射Tau,连续3 d,于末次给药30 min后结扎冠状动脉。通过MS2000计算机生物信号采集分析系统记录大鼠心脏左室压(LVDP)、左室压力升高/降低最大速率(±dp/dtmax);采用ELISA方法检测血清心肌肌钙蛋白I(cTnI)、肌酸激酶同工酶(CK-MB)、超氧化物歧化酶(SOD)和丙二醛(MDA);NBT法计算心肌梗死面积百分比。结果 Tau(200、400 mg·kg-1)预处理均能够改善心肌梗死后大鼠心脏功能(P<0.05);Tau(100、200、400 mg·kg-1)预处理均降低血清cTnI及CK-MB值(P<0.05),升高血清SOD活性、降低MDA含量(P<0.01或P<0.05),减少心肌梗死面积(P<0.01或P<0.05),且其作用呈一定的剂量依赖性。结论 Tau对大鼠心肌梗死有保护作用,其机制可能与减少自由基生成,抗氧化损伤有关。

Protective effects of taurine preconditioning on myocardial infarction in rats

Aim To observe the cardioprotective effect of taurine preconditioning in the rat model of myocardial infarction.Methods 40 male Wistar rats were randomly divided into 5 groups:control group(CON),myocardial infarction group(MOD),taurine low-dose group(100 mg·kg-1),taurine medium-dose group(200 mg·kg-1),taurine high-dose group(400 mg·kg-1).Left ventricular developed pressure(LVDP) and ±dp/ dt max were recorded.Serum cardiac Troponin I(cTnI),creatine kinase isoenzyme(CK-MB),Superoxide Dismutase(SOD) and Malondialdehit(MDA) were mornitored.Myocardial infarct size was measured with NBT staining method.Results Compared with MOD group,taurine group resulted in a significantly improved functional recovery.LVDP,± dp/dt max(P<0.05) and serum SOD level were significantly mcreased.cTnI,CK-MB,MDA and infarct size(P<0.01) were significantly decreased.Meanwhile,the protective effect was in a taurine dose dependent manner.Conclusion Taurine preconditioning has a protective and dose-dependent effect on myocardial infarction in rats.The mechanism is related to the reduction of the oxidative damage.