翻转肠囊法研究芒果苷在大鼠的肠吸收动力学

目的考察芒果苷在大鼠不同肠段的吸收特性及知母、知母-黄柏、P-糖蛋白抑制剂对芒果苷肠吸收的影响。方法采用大鼠肠外翻模型,UPLC法测定不同时间点肠囊内芒果苷浓度,寻找芒果苷在肠中吸收的最佳部位;观察知母、知母-黄柏及P-糖蛋白抑制剂对芒果苷吸收的影响。结果芒果苷在小肠的吸收为回肠>空肠>结肠;在回肠段知母水煎液中芒果苷90 min累积吸收量(Q90)、转运速率(Vt)和表观渗透系数(Paap)均大于芒果苷单体组和知母-黄柏水煎液组(P<0.05);环孢素A提高芒果苷在回肠K氏液中的浓度(P<0.05)。结论芒果苷在不同肠段的吸收有差异;知母促进了芒果苷的肠吸收;芒果苷可能是P-gp底物。

Study on intestinal absorption of mangiferin by everted gut sacs model

Aim To investigate the absorption of mangiferin in different rat intestines,and the effects of Pgp inhibitors,Zhimu and Zhimu-Huangbai on mangiferin absorption.Methods The rat everted gut sacs model was used.Mangiferin in samples was determined by UPLC method,and the absorption of mangiferin at different intestinal segments was observed.The effects of P-gp inhibitors,Zhimu and Zhimu-Huangbai on the absorption of mangiferin had been studied.Results The descending order of mangiferin absorption was ileum,colon and jejunum.Q 90,V t and P aap of mangiferin in Zhimu group at ileum were significantly higher than those in mangiferin compound group and ZhimuHuangbai group.The absorption of mangiferin was significantly increased while combined with cyclosporine A.Conclusions There is difference on the absorption rate of mangiferin in different intestines;Zhimu increases the absorption of mangiferin;mangiferin may be the substrate of P-gp.