一测多评法测定南方菟丝子中7种活性成分的含量

目的建立同时测定南方菟丝子Cuscuta australis中绿原酸、隐绿原酸、咖啡酸、金丝桃苷、异槲皮苷、紫云英苷、山柰酚的一测多评法,验证该方法在南方菟丝子质量分析中应用的科学性及可行性。方法采用高效液相色谱法,以金丝桃苷为内参物,建立该成分与绿原酸、隐绿原酸、咖啡酸、异槲皮苷、紫云英苷和山柰酚的相对校正因子,采用相对校正因子来计算各成分的质量分数,同时用外标法测定南方菟丝子及其炮制品中7种活性成分的质量分数,对南方菟丝子一测多评的计算结果与外标法实测值进行比较,评价一测多评法在南方菟丝子中应用的准确性和科学性。结果各相对校正因子重复性良好,一测多评法测定结果与外标法测定结果无显著差异。结论以金丝桃苷为内标同时测定绿原酸、隐绿原酸、咖啡酸、异槲皮苷、紫云英苷、山柰酚的一测多评法可用于南方菟丝子的定量分析。

Determination of seven effective constituents in crude and processed Cuscuta australis by quantitative analysis of multi-components with single marker

Objective To establish a quantitative analysis of multi-components with single marker (QAMS) for the simultaneous determination of chlorogenic acid, cryptochlorogenin acid, caffeic acid, hyperoside, isoquercitrin, astragalin, kaempferol in crude and processed Cuscuta australis, which is proved to be a scientific and feasible method in the quality analysis in C. australis. Methods Six relative correction factors (RCFs) of chlorogenic acid, cryptochlorogenin acid, caffeic acid, isoquercitrin, astragalin, kaempferol was established in the HPLC method with the hyperoside as the internal standard (IS), which was to calculate the mass fraction of each. The mass fraction of seven effective constituents in crude and processed C. australis was calculated by the external standard method (ESM) at the same time. Compared with the content results determined by the ESM and QAMS, the feasibility and accuracy of QAMS method were verified. Results The relative correction factor (RCF) was perfect. The detection calculated by QAMS was consistent with the results by ESM. Conclusion The method with a single marker, using the hyperoside as IS, is accurate and feasible for the quantitative analysis of six other effective constituents in C. australis.