| 丹参潜在性致病内生真菌的筛选及其致病相关酶酶学特性分析 |
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| 引用本文： | 林亚丽,黄倩倩,严铸云,陈新,王海,何冬梅,韩桂琪.丹参潜在性致病内生真菌的筛选及其致病相关酶酶学特性分析[J].中国实验方剂学杂志,2018,24(4):38-43. |
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| 作者姓名： | 林亚丽 黄倩倩 严铸云 陈新 王海 何冬梅 韩桂琪 |
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| 作者单位： | 成都中医药大学 中药材标准化省部共建教育部重点实验室, 四川省中药资源系统研究与开发利用省部共建国家重点实验室, 成都 611137,成都中医药大学 中药材标准化省部共建教育部重点实验室, 四川省中药资源系统研究与开发利用省部共建国家重点实验室, 成都 611137,成都中医药大学 中药材标准化省部共建教育部重点实验室, 四川省中药资源系统研究与开发利用省部共建国家重点实验室, 成都 611137,成都中医药大学 中药材标准化省部共建教育部重点实验室, 四川省中药资源系统研究与开发利用省部共建国家重点实验室, 成都 611137,成都中医药大学 中药材标准化省部共建教育部重点实验室, 四川省中药资源系统研究与开发利用省部共建国家重点实验室, 成都 611137,成都中医药大学 中药材标准化省部共建教育部重点实验室, 四川省中药资源系统研究与开发利用省部共建国家重点实验室, 成都 611137,成都中医药大学 中药材标准化省部共建教育部重点实验室, 四川省中药资源系统研究与开发利用省部共建国家重点实验室, 成都 611137 |
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| 基金项目： | 国家自然科学基金项目（81573537） |
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| 摘 要： | 目的:从18株丹参内生真菌中筛选具有潜在性致病作用的内生真菌,并对其致病机制进行探究。方法:采用内生真菌与组培苗共培养方式,根据组培苗的发病情况筛选出潜在性致病作用的内生真菌,测定活体外和活体根内的纤维素酶羧甲基纤维素酶(Cx),β-葡萄糖苷酶(β-G)],果胶酶多聚半乳糖醛酸酶(PG),聚甲基半乳糖醛酸酶(PMG)]和半纤维素酶(木聚糖酶)等细胞壁降解酶(CWDE)活性的变化。结果:在活体外,PG,PMG,木聚糖酶活性先增加后降低到后期基本不变,Cx,β-G酶活性随着培养时间的增加而持续升高。在活体内,9株潜在性致病内生真菌均能在组培苗根部组织产生细胞壁降解酶,但不同菌种差异较大。其中7株菌接种的组培苗根部Cx,β-G活性显著高于未接菌的根部(P0.05),6株菌接种的组培苗根部PG活性显著高于未接菌的根部(P0.05),9株菌接种的组培苗根部PMG以及木聚糖酶活性显著高于未接菌的根部(P0.05)。结论:Cx,β-G和PG,PMG以及木聚糖酶在潜在性致病内生菌的致病过程中起到了重要作用,是重要的致病因子,但各潜在性致病内生真菌的作用环节存在差异。
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| 关 键 词： | 丹参 潜在性致病内生真菌 细胞壁降解酶 |
| 收稿时间： | 2017/8/18 0:00:00 |
Screening of Potentially Pathogenic Endophytic Fungi of Salvia miltiorrhiza and Analysis of Their Relevant Enzymatic Characteristics |
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| LIN Ya-li,HUANG Qian-qian,YAN Zhu-yun,CHEN Xin,WANG Hai,HE Dong-mei and HAN Gui-qi.Screening of Potentially Pathogenic Endophytic Fungi of Salvia miltiorrhiza and Analysis of Their Relevant Enzymatic Characteristics[J].China Journal of Experimental Traditional Medical Formulae,2018,24(4):38-43. |
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| Authors: | LIN Ya-li HUANG Qian-qian YAN Zhu-yun CHEN Xin WANG Hai HE Dong-mei and HAN Gui-qi |
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| Institution: | Chengdu University of Traditional Chinese Medicine(TCM), Key Laboratory of Standardization of Traditional Chinese Medicinal Herbs Co-sponsored by Province and Ministry of Education, Key State Key Laboratory and Breeding Base of systematic research, Development and Utilization of TCM Resource Co-sponsored by Province and Ministry of Education, Chengdu 611137, China,Chengdu University of Traditional Chinese Medicine(TCM), Key Laboratory of Standardization of Traditional Chinese Medicinal Herbs Co-sponsored by Province and Ministry of Education, Key State Key Laboratory and Breeding Base of systematic research, Development and Utilization of TCM Resource Co-sponsored by Province and Ministry of Education, Chengdu 611137, China,Chengdu University of Traditional Chinese Medicine(TCM), Key Laboratory of Standardization of Traditional Chinese Medicinal Herbs Co-sponsored by Province and Ministry of Education, Key State Key Laboratory and Breeding Base of systematic research, Development and Utilization of TCM Resource Co-sponsored by Province and Ministry of Education, Chengdu 611137, China,Chengdu University of Traditional Chinese Medicine(TCM), Key Laboratory of Standardization of Traditional Chinese Medicinal Herbs Co-sponsored by Province and Ministry of Education, Key State Key Laboratory and Breeding Base of systematic research, Development and Utilization of TCM Resource Co-sponsored by Province and Ministry of Education, Chengdu 611137, China,Chengdu University of Traditional Chinese Medicine(TCM), Key Laboratory of Standardization of Traditional Chinese Medicinal Herbs Co-sponsored by Province and Ministry of Education, Key State Key Laboratory and Breeding Base of systematic research, Development and Utilization of TCM Resource Co-sponsored by Province and Ministry of Education, Chengdu 611137, China,Chengdu University of Traditional Chinese Medicine(TCM), Key Laboratory of Standardization of Traditional Chinese Medicinal Herbs Co-sponsored by Province and Ministry of Education, Key State Key Laboratory and Breeding Base of systematic research, Development and Utilization of TCM Resource Co-sponsored by Province and Ministry of Education, Chengdu 611137, China and Chengdu University of Traditional Chinese Medicine(TCM), Key Laboratory of Standardization of Traditional Chinese Medicinal Herbs Co-sponsored by Province and Ministry of Education, Key State Key Laboratory and Breeding Base of systematic research, Development and Utilization of TCM Resource Co-sponsored by Province and Ministry of Education, Chengdu 611137, China |
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| Abstract: | |
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| Keywords: | Salvia miltiorrhiza potentially pathogenic endophytic fungi cell wall degrading enzyme |
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