益气活血解毒方对小鼠卵巢癌ID-8细胞株增殖及血管生成过程的影响

目的:研究益气活血解毒方抑制鼠卵巢上皮癌ID-8细胞株增殖的作用,并观察本方对于脐静脉内皮细胞(HUVECs)形成血管过程的作用,为本方发挥疗效提供实验依据。方法:采用细胞增殖与检测方法(cell counting kit-8,CCK-8)检测本方对于ID-8细胞增殖的抑制作用;将含中药血清、磁珠分选的调节性T细胞(regulatory T cells,Tregs)与抗血管内皮生长因子(VEGF)抗体分组共培养,观察血管生成情况差异。结果:与空白组比较,10%,20%含药血清均能够抑制卵巢上皮癌ID-8细胞株的增殖,此抑制作用在培养96 h时最明显;在72,96 h时,20%含药血清组的抑制率明显高于10%含药血清组(P0.05,P0.01)。与Tregs(-)各组相比,Tregs(+)各组HUVECs细胞呈现明显的血管网状结构,中药含药血清组血管成网不明显,VEGF抗体联合含药血清组血管成网情况最差。结论:益气活血解毒方对小鼠卵巢上皮癌ID-8细胞株的增殖过程有显著的抑制作用,同时能够干预血管的形成,这也许与本方影响Tregs促进肿瘤新生血管的作用相关。

Effect of Yiqi Huoxue Jiedu Decoction on Proliferation of Ovarian Cancer ID-8 Cells and Angiogenesis

Objective: To analyze the inhibitory effects of Yiqi Huoxue Jiedu decoction on proliferation of ovarian epithelial carcinoma ID-8 cells in vitro, observe the effect of this method on angiogenesis of human umbilical vein endothelial cells (HUVECs), and provide experimental basis for its efficacy. Method: Cell counting kit-8 (CCK-8) was used to testify the inhibitory effect of Yiqi Huoxue Jiedu decoction on the proliferation of ovarian cancer ID-8 cells; decoction serum, regulatory T cells (Tregs) isolated by immune magnetic MACS MicroBeads, as well as vascular endothelial growth factors (VEGF) were co-cultured, and then the angiogenesis of HUVEC was observed. Result: As compared with blank control group (fetal bovine serum group), drug serum groups with concentration of 10% and 20% showed inhibitory effects on ID-8 cells, and this effect was most significant at 96 h. Serum group with concentration of 20% showed remarkable advantages against 10% group at 72 h (P<0.01) and 96 h (P<0.05). HUVECs cultivated with Treg cells exhibited a better angiogenesis process. On the other hand, the vascular network was not obvious in drug serum group, and the inhibitory effect was most significant in anti-VEGF+Yiqi Huoxue Jiedu decoction. Conclusion: Yiqi Huoxue Jiedu decoction can restrict the multiplication of ovarian epithelial carcinoma ID-8 cells, and it could intervene the angiogenesis of HUVECs, which is probably associated with the intervention on Tregs.