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Effect of calcium on proteolytic activity and conformation of hemorrhagic toxin I from five pace snake (Agkistrodon acutus) venom |
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| Authors: | Jiajie Zhang Zhenxie Chen Youzhao He Xun Xu |
| Institution: | a Department of Biology University of Science and Technology of China, Hefei, Anhui, The People's Republic of China b Department of Chemistry, University of Science and Technology of China, Hefei, Anhui, The People's Republic of China |
| Abstract: | J. J. Zhang, Z. X. Chen, Y. Z. He and X. Xu. Effect of calcium on proteolytic activity and conformation of hemorrhagic toxin I from five pace snake (Agkistrodon acutus) venom. Toxicon22, 931–935, 1984. — AaHI, a proteolytic hemorrhagic toxin from A. acutus venom, contains one g-atom Zn per mole protein and 2 g-atoms Ca per mole protein. AaHI is activated by calcium and slightly inhibited by zinc. When treated with EDTA, AaHI is completely inactivated. When dialyzed against 1,10-O-phenanthroline, 50–80% of the metal content and activities are lost, while 90% of the hemorrhagic and proteolytic activities are retained when dialyzed against 1,10-O-phenanthroline containing 5mM Ca2+. The results suggest that calcium is essential for the hemorrhagic and proteolytic activities. The circular dichroism spectra show that calcium may play an important role in maintaining a proper structure for AaHI. The function of zinc is not clear. |
| Keywords: | To whom reprint requests should be addressed |
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