Tubuloreticular Inclusions and Paired Cisternae Induced in Human Lymphocytes Cultured with Staphylococcus Aureus Cowan 1 |
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Authors: | Jun Kuyama Yoshio Kanayama Shuichi Katagiri Toshiharu Tamaki Takeshi Yonezawa Seiichiro Tarui |
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Affiliation: | a The Second Department of Internal Medicine, Osaka University Medical School, Fukushima-ku, Osaka, Japan |
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Abstract: | Tubuloreticular inclusions (TRI) and paired cisternae (PC) were induced in lymphocytes of normal individuals after incubation with Staphylococcus aureus Cowan 1. TRI were initially detected in lymphoid cells on day 2 (48-h culture). The frequency of TRI-positive cell sections on day 5 increased about twofold over those on days 2-4. On day 7, TRI were predominantly seen in lymphoplasmacytoid cells or plasmacytoid cells, with an incidence of up to 18% of sections. The regions in these cells were most extensive and anastomosed with the cisternae of adjacent well-developed rough endoplasmic reticulum (RER). TRI formation appears not to be essential for mitogen-induced B-cell differentiation to plasmacytoid cells, because poke-weed mitogen (PWM) failed to induce TRI. The diverse expressions of TRI induction between these two mitogens may be due to a difference in B-cell activation mechanisms.
Paired cisternae were observed in a great majority of mitotic cells at various stages. These were encountered most frequently on day 4. PC were also seen in the PWM-stimulated culture. Our observations suggest that PC formation may be related to new formation of RER as well as to reconstruction of the nuclear envelope. |
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Keywords: | tubuloreticular inclusions paired cisternae Staphylococcus aureus Cowan 1 Interferon lymphocytes plasma cells |
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