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A novel method for the serum-free plating of stage-24 chick limb bud cells |
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| Authors: | Sheila S. Southerland Paul A. Lucas PhD |
| Affiliation: | (1) Department of Surgery, Mercer University School of Medicine at the Medical Center of Central Georgia, Macon, Georgia, USA;(2) Department of Surgery, Medical Center of Central Georgia, 777 Hemlock Street, Hospital Box 140, 31208-6000 Macon, GA, USA |
| Abstract: | Summary A modification of the standard plating of Stage 24 chick limb bud cells in serum-containing media (SCM) is described in which the cells are incubated in SCM for 3 hours before direct plating in a serum-free chemically defined media (SFM). This results in equal plating efficiencies to those obtained with plating in SCM and may allow for more defined culture conditions when testing mitogenic growth factors found in serum. |
| Keywords: | Defined media Growth factors Mitogenesis Plating Serum containing media Serum-free media Stage 24 chick cells |
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