半夏泻心汤及其拆方对胃腺癌SGC-7901细胞周期和凋亡的影响

目的:从细胞增殖、凋亡、细胞周期角度,研究半夏泻心汤治疗胃癌机制。方法:把半夏泻心汤拆分为苦降、辛开和补益3个组方,1×104细胞/孔接种于96孔板,药物浓度为4,2,0.5,0.125 g·L-1,MTT法检测细胞增殖;1×105/孔接种于6孔板,全方和苦降高、低浓度为0.5,0.125 g·L-1,辛开和补益高、低浓度为4,2 g·L-1,流式细胞仪检测细胞周期和细胞凋亡。观察半夏泻心汤及其拆方对胃癌细胞SGC-7901增殖、细胞周期和凋亡的影响,并与5-氟尿嘧啶(5-Fluorouracil,5-Fu)比较。结果:半夏泻心汤全方及苦降、辛开组方能够抑制SGC-7901细胞增殖,全方IC50为1.645 g·L-1,苦降组为1.446 g·L-1,辛开组为3.867 g·L-1;它们增加SGC-7901细胞S期和G0-G1期百分比,促进细胞凋亡,高浓度强于低浓度,但补益组方对此没有作用。结论:半夏泻心汤及其拆方能够一定程度的抑制细胞增殖,以苦降组为最,补益组作用甚微,其机制可能与药物阻滞细胞至G1/S期和诱导细胞凋亡有关。

Effects of Banxia Xiexin Decoction and its Components on Cell Cycle and Apoptosis of Gastric Cancer SGC-7901 Cells

Objective: Study the effects and mechanism of Banxia Xiexin decoction on gastric cancer. Method: Banxia Xiexin decoction was divided into Pungent openning(dried ginger,pinellia ternate)group,Bitter Descending(coptis chinensis,scutellaria) group and Invigoration(glycyrrhiza,ginseng) group by its different nature and flavor. 1×10⁴cells/wells were planted at 96 wells plate, drug concentrations were 4, 2, 0.5, 0.125 g·L^-1, cell proliferation was detected by MTT method. 1×10⁵ cells/wells were planted in 6 wells plate. The high and low concentration of whole Party and Bitter Descending drugs was respectively 0.5, 0.125 g·L^-1. The high and low concentration of Pungent openning and invigoration was respectively 4,2 g·L^-1. Effect of Banxia Xiexin decoction and compositions on gastric cancer SGC-7901 cells proliferation, cell cycle and apoptosis ratio were detected and compared with known positive drug 5-fluorouracil (5-FU). Result: Banxia Xiexin decoction group, Bitter Descending group and Pungent opening group could inhibit the proliferation of SGC-7901 cell, the IC₅₀ values of its were 1.645, 1.446, 3.867 g·L^-1 respectively, and increased SGC-7901 cells in S phase and G₀-G₁ phase percentage with promoting apoptosis in dose-dependent manner, but invigoration group had no such effect. Conclusion: Banxia Xiexin decoction and its compositions can inhibit the proliferation of SGC-7901 cell, Bitter Descending is strongest and Invigoration group is the weakest,which may be related to blocking of cells to G₁/S phase and inducing cell apoptosis