活血、破血药对急性脑缺血大鼠基底动脉内皮细胞VEGF和bFGF表达的影响

目的:探讨活血、破血药对急性脑缺血动脉血管内皮细胞生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)表达的影响。方法:SD大鼠40只,随机分为假手术组、模型组、活血组、破血组,每组10只;假手术组予以假手术,其余各组结扎法制作大鼠急性脑缺血模型。造模前每天上午9时预防ig给药7 d,假手术组、模型组ig蒸馏水20 mL·kg-1,活血组ig当归、川芎煎液20 mL·kg-1(相当于3.6 g·kg-1),破血组ig三棱、莪术煎液20 mL·kg-1(相当于1.8 g·kg-1)。造模观察2 d后如前法继续给药7 d,2 h后静脉空气栓塞处死,取基底动脉组织。免疫组化法检测基底动脉内皮细胞VEGF和bFGF表达。结果:与模型组VEGF平均吸光度(A,0.348±0.086)比较,活血组A(0.443±0.061)、破血组A(0.558±0.12)VEGF蛋白表达升高,差异具有统计学意义(P<0.01～P<0.05);活血组与破血组间比较,破血组VEGF蛋白表达更强,差异具有统计学意义(P<0.05)。与模型组bFGF(0.394±0.080)比较,活血组bFGF(0.553±0.097)蛋白表达升高,差异具有显著统计学意义(P<0.01),破血组bFGF(0.302±0.084)蛋白表达降低,差异具有统计学意义(P<0.05)。结论:活血、破血药均能促进VEGF蛋白的表达,而且破血药作用更强;活血药可升高bFGF蛋白的表达,破血药却能降低bFGF蛋白的表达。这说明两者在活血化瘀的机制上既有相同之处,又有差异。

Promoting and Expelling Blood Circulation Drugs Affect the Expression of VEGF and bFGF of Endothelial Cells in Acute Cerebral Ischemia Basilar Artery

Objective: To investigate the promoting and expelling blood circulation drugs on the expression of vascular endothelial cell growth factor (VEGF) into basic fibroblast growth factor (bFGF) in acute cerebral ischemic arterial. Method: Forty SD rats were randomly divided into sham operation group, model group, promoting blood group, expelling blood group(n=10). The acute cerebral ischemia model was established. The sham operation and model groups were given distilled water, promoting blood group was given Angelica and Chuanxiong decoction 3.6 g·kg^-1, expelling group was given Sanleng Ezhu decoction 1.8 g·kg^-1.Drugs were given before modeling for 7 days, after 2 days of modeling,continuous administration for 7 day was carried out of 2 h after end administration, rats were killed for collection of the basilar artery. Immunohistochemical was used for detection of basilar artery endothelial cells, VEGF and bFGF expression. Result: Compared with model group VEGF absorbance (A) of (0.348±0.086), in promoting blood group (0.443±0.061) and expelling blood group (0.558±0.12), VEGF protein expression was increased in a statistically significant difference (P<0.01 to P<0.05). Compared with promoting blood group, the effect of expelling blood group was stronger, the difference was statistically significant (P<0.05). Compared with the model group A of bFGF (0.394±0.080), in promoting blood group bFGF (0.553± 0.097) protein expression was elevated, the difference was statistically significant (P<0.01), in the expelling blood group bFGF (0.302±0.084) protein expression decreased, the differences statistically significant (P<0.05). Conclusion: Promoting blood circulation drugs and expelling blood circulation drugs can promote the expression of VEGF protein, and expelling the drug showed more potent; promoting blood circulation drugs increased the expression of bFGF protein, expelling blood circulation drugs were able to reduce the expression of bFGF protein. This shows that for the mechanism of promoting blood circulation,promoting and expelling blood circulationdrugs have both similarities and differences.