冠心苏合胶囊对急性心肌梗死大鼠肌钙蛋白T及心肌酶的影响

目的:利用心肌梗死大鼠模型观察肌钙蛋白T(cTn-T)在心肌损伤中的动态变化并确定其达峰时间,进而观察冠心苏合胶囊在这时间点上对急性心肌梗死大鼠cTn-T及心肌酶的影响。方法:实验分为两个阶段,第一阶段(摸索cTn-T达峰时间):大鼠经结扎左冠状动脉前降支造成心肌梗死,分别在结扎后12,31,48,69 h检测cTn-T含量并确定其达峰时间。实验第二阶段(冠心苏合作用观察):取正常大鼠随机分为假手术对照组、模型对照组和卡托普利阳性药对照组,以及冠心苏合胶囊0.8,0.4 g·kg-1剂量组,均灌胃给药,每天1次,连续5 d,于第5天给药后1 h制备大鼠心肌梗死模型,存活大鼠给药治疗1次,在cTn-T达峰时间点测定各组大鼠cTn-T含量,测定各组大鼠天冬氨酸转氨酶(AST)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)等血清心肌酶,NBT心脏染色测定心肌梗死质量百分比,术前、结扎即刻、结扎31 h测心电图,取大鼠左心室用福尔马林固定进行组织病理学检查。结果:①cTn-T达峰时间:通过12,31,48,69 h几个时间点cTn-T含量比较,大鼠左冠状动脉结扎31,48 h模型组与假手术组cTn-T含量有显著性差异(P<0.001),确定结扎31 h为cTn-T出现高峰时间点;②在对冠心苏合胶囊的药效观察实验中,取31 h作为观察时间点,可见冠心苏合胶囊高剂量组大鼠血清中cTn-T和AST含量下降,与模型组比较差异有统计学意义(P<0.01,P<0.01)。③对心肌梗死质量百分比的影响,冠心苏合胶囊高剂量组大鼠心肌梗死质量比低于模型组,差异有统计学意义(P<0.05)。④对心电图的影响,在结扎即刻和结扎31 h冠心苏合高、低剂量组ST段抬高明显低于模型组;⑤心脏HE染色发现冠心苏合两剂量组心肌组织病理变化均减轻,其中,冠心苏合高剂量组减轻更为明显。结论:在急性心肌梗死大鼠模型中,cTn-T表现出特异性和敏感性,并在31 h最接近达峰时间;冠心苏合胶囊能在该时间点降低心肌梗死大鼠cTn-T,AST含量,降低心肌梗死大鼠梗死心肌质量百分比。

Effect of Guanxin Suhe Capsule on cTn-T and Myocardial Enzyme in Rat Acute Myocardial Infarction

Objective: To evaluate the dynamic changing of troponin(cTn-T) and observe the treatment effect of Guanxin Suhe capsule on acute myocardial infarction(AMI) in rats and investigate its mechanism. Method: Rats were selected to establish acute myocardial infarction model. The rats were divided randomly into five groups and treated with pure water,high-dose Guanxin Suhe (0.8 g·kg^-1),low-dose Guanxin Suhe (0.4 g·kg^-1),captopril respectively for 5 days, then AMI model were established. cTn-T,aspartate aminotransferase(AST),creatine kinase enzyme(CK), MBisoenzyme of creatine kinase(CK-MB) was determined after ligating 31 h. Result: The area of myocardial infarction in the Guanxin Suhe capsule high dosage group was reduced, cTn-T and AST decreased, showing a statistical significance compared with the model group (P<0.05, P<0.01,P<0.01).The effect of high-dose and low-dose Guanxin Suhe capsule on ECG in acute myocardial infarction rats was observed, the heights of ST segment were reduced just after ligating 0 h and 31 h. Conclusion: Guanxin Suhe capsule has significant effect on myocardial infarction, it treats myocardial infarction through regulating cTn-T and AST.