荞麦花叶提取物抗氧化活性与抑瘤作用的实验研究

目的:研究荞麦花叶提取物(extraction of buckwheat flower and leaf,EBFL)对肿瘤的抑制作用及其对荷瘤鼠体内抗氧化能力的影响。方法:建立S180小鼠实体瘤和腹水瘤模型,80只昆明种小鼠随机分为4组:模型组、EBFL 200,400 mg·kg-1剂量组ig给药、环磷酰胺(CTX,25 mg·kg-1)ip组。所有动物每日给药1次,连续给药20 d,测定EBFL给药后各处理组小鼠实体瘤重,腹水瘤小鼠体重和腹围增长速度,治疗组和对照组观察时间结点为25 d,计算各组平均生存率,以及全血谷胱甘肽过氧化物酶(GSH-Px)及血浆超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:EBFL处理15 d,荷瘤小鼠腹水增长、体重增长明显减慢,与模型组比较P<0.05。EBFL处理组腹水瘤小鼠生存率达25%。EBFL对肿瘤的生长有一定的抑制作用,但仍未达到CTX的水平。与对照组相比,EBFL(L,H)均可升高荷瘤小鼠血清中GSH-Px活性(P<0.05,P<0.01);EBFL(H)组小鼠血清SOD活性与对照组相比显著升高(P<0.05),作用效果与CTX相似;与对照组相比,EBFL(L,H)处理均可以显著降低小鼠血清MDA水平(P<0.05)。结论:荞麦花叶提取物对S180荷瘤小鼠有一定的抑制作用,能抑制荷瘤小鼠腹水生长,延长荷瘤小鼠生存率,这可能与提高实验小鼠体内的GSH-Px和SOD的活性,降低MDA含量有关。

Study on Antioxidative and Antitumor Effect of Extraction of Buckwheat Flower and Leaf

Objective: To explore the antioxidative and antitumor effects of buckwheat flower and leaf extracts on S180 tumor-bearing mice. Method: The mice S180 tumor model was established; the model groups were divided into extraction of buckwtheal flower and leaf(EBFL)-low group (200 mg·kg^-1), EBFL-high group (400 mg·kg^-1), and combined with cydophosphamide(CTX,25 mg·kg^-1). The solid tumor weight, ascites tumor weight and abdominal circumference growth rate and survival rate were measuredafter administration. glutathione peroxidase(GSH-Px), superoxide dismutase(SOD) activity and malondialdehyde(MDA) content were determined. Result: After EBFL treatment for 15 d,the symptom of body weight loss was ameliorated and the growth of tumor was inhibited in tumor-bearing mice.EBFL(L,H)treatment could increase the levels of serum GSH-Px (P<0.05,P<0.01) and the activity of SOD in EBFL(H)group was obviously increased (P<0.05) compared with those of control group. The effect was similar to CTX. Compared with control group, the level of MDA was decreased significantly in tumor-bearing mice. Conclusion: EBFL can inhibit the growth of S180 mouse in a certain extent, which may be related to the increasing GSH-Px and SOD activity and decreased MDA content.